SARS-CoV-2 Envelope Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-07060
![ELISA: SARS-CoV-2 Envelope Antibody - BSA Free [NBP3-07060]](https://resources.bio-techne.com/images/products/SARS-CoV-2-Envelope-Antibody-ELISA-NBP3-07060-img0004.jpg "ELISA: SARS-CoV-2 Envelope Antibody - BSA Free [NBP3-07060]")
![Western Blot: SARS-CoV-2 Envelope AntibodyBSA Free [NBP3-07060]](https://resources.bio-techne.com/images/products/SARS-CoV-2-Envelope-Antibody-Western-Blot-NBP3-07060-img0002.jpg "Western Blot: SARS-CoV-2 Envelope AntibodyBSA Free [NBP3-07060]")
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
SARS-CoV-2
Predicted:
SARS-CoV (93%). Backed by our 100% Guarantee.
Applications
Immunohistochemistry, Western Blot, ELISA
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
This antibody was raised against a peptide corresponding to 14 amino acids near the carboxyl terminus of SARS-CoV-2 (COVID-19) Envelope protein. The immunogen is located within the last 50 amino acids of SARS-CoV-2 (COVID-19) Envelope protein.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for SARS-CoV-2 Envelope Antibody - BSA Free
Western Blot: SARS-CoV-2 Envelope AntibodyBSA Free [NBP3-07060]
Western Blot: SARS-CoV-2 Envelope Antibody [NBP3-07060] - Loading: 50 ng per lane of SARS-CoV-2 (COVID-19) Envelope recombinant protein. Antibodies: SARS-CoV-2 (COVID-19) Envelope,1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1: 1 ug/mL and Lane 2: 2 ug/mL![Immunohistochemistry: SARS-CoV-2 Envelope Antibody - BSA Free [NBP3-07060]](https://resources.bio-techne.com/images/products/SARS-CoV-2-Envelope-Antibody-Immunohistochemistry-NBP3-07060-img0001.jpg "Immunohistochemistry: SARS-CoV-2 Envelope Antibody - BSA Free [NBP3-07060]")
Immunohistochemistry: SARS-CoV-2 Envelope Antibody - BSA Free [NBP3-07060]
Immunohistochemistry: SARS-CoV-2 Envelope Antibody [NBP3-07060] - Immunohistochemistry analysis of paraffin-embedded COVID-19 patient lung tissue using (0.5 ug/mL). Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4 degrees celsius. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin. Strong signal of SARS-COV-2 envelope protein was observed in macrophage of COVID-19 patient lung, but not in non-COVID-19 patient lung.![ELISA: SARS-CoV-2 Envelope Antibody - BSA Free [NBP3-07060]](https://resources.bio-techne.com/images/products/SARS-CoV-2-Envelope-Antibody-ELISA-NBP3-07060-img0003.jpg "ELISA: SARS-CoV-2 Envelope Antibody - BSA Free [NBP3-07060]")
ELISA: SARS-CoV-2 Envelope Antibody - BSA Free [NBP3-07060]
ELISA: SARS-CoV-2 Envelope Antibody [NBP3-07060] - Antbodies: SARS-CoV-2 Envelope Antibody. A direct ELISA was performed using SARSCoV-2 Envelope recombinant protein (92-730) as coating antigen and the anti-SARS-CoV-2 (COVID-19) Envelope antibody as the capture antibody. Secondary: Goat an-rabbit IgG HRP conjugate at 1:20000 diluon. Detection range is from 1 ng/mL to 3000 ng/mLApplications for SARS-CoV-2 Envelope Antibody - BSA Free
Application
Recommended Usage
ELISA
1 ug/mL
Immunohistochemistry
0.5 ug/mL
Formulation, Preparation, and Storage
Purification
Peptide affinity purified
Formulation
PBS
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: SARS-CoV-2 Envelope
References
1. Malik Y. A. (2020). Properties of Coronavirus and SARS-CoV-2. The Malaysian Journal of Pathology.
2. Yoshimoto F. K. (2020). The Proteins of Severe Acute Respiratory Syndrome Coronavirus-2 (SARS CoV-2 or n-COV19), the Cause of COVID-19. The Protein Journal. https://doi.org/10.1007/s10930-020-09901-4
3. Uniprot (P0DTC4)
4. J Alsaadi, E. A., & Jones, I. M. (2019). Membrane binding proteins of coronaviruses. Future Virology. https://doi.org/10.2217/fvl-2018-0144
Alternate Names
2019-nCoV Envelope protein, COVID-19 Envelope protein, E Protein, Envelope, Human Coronavirus Envelope protein, SARSCoV2, SARS-CoV-2 E protein, SARSCoV2 Envelope protein, SARS-CoV-2 Envelope protein, Severe acute respiratory syndrome 2 Envelope Protein
Gene Symbol
E
Additional SARS-CoV-2 Envelope Products
Product Documents for SARS-CoV-2 Envelope Antibody - BSA Free
Product Specific Notices for SARS-CoV-2 Envelope Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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