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Key Product Details

Species Reactivity

Validated:

SARS-CoV-2

Applications

ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

Product Summary for SARS-CoV-2 3CL Protease Antibody - BSA Free

Immunogen

This antibody was raised against a peptide corresponding to 15 amino acids in the center of SARS-CoV-2 (COVID-19) 3CL-PRO (NSP5) protein. The immunogen is located within 80-130 amino acids of the SARS-CoV-2 (COVID-19) 3CL-PRO (NSP5) protein.

Reactivity Notes

Predicted reactivity based on immunogen sequence: SARS-CoV 3CL-PRO (NSP5) protein: (87%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SARS-CoV-2 3CL Protease Antibody - BSA Free

Western Blot: SARS-CoV-2 3CL Protease Antibody [NBP3-07061] - Western Blot Validation with SARS-CoV-2 3CL Protease Protein. Loading: 30 ng per lane of SARS-CoV-2 3CL Protease recombinant protein (10-400). Antibodies: SARS-CoV-2 3CL Protease (NBP3-07061), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1: 0.5 ug/ml and Lane 2: 1 ug/ml
ELISA: SARS-CoV-2 3CL Protease Antibody [NBP3-07061] - ELISA Validation with SARS-CoV-2 3CL Protease Protein . Antibodies: SARS-CoV-2 3CL Protease Antibody, NBP3-07061. A direct ELISA was performed using SARS-CoV-2 3CL-PRO (NSP5) recombinant protein as coating antigen and the anti-SARS-CoV-2 3CL Protease antibody as the capture antibody. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution. Detection range is from 1 ng/mL to 3000 ng/mL

Applications for SARS-CoV-2 3CL Protease Antibody - BSA Free

Application
Recommended Usage

ELISA

1 ug/mL

Western Blot

0.5 ug/ml
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Published Applications

Read 1 publication using NBP3-07061 in the following applications:

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: 3CL Protease

Long Name

3CL Protease

Alternate Names

Main Protease, Mpro

Gene Symbol

ORF1ab

Additional 3CL Protease Products

Product Documents for SARS-CoV-2 3CL Protease Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for SARS-CoV-2 3CL Protease Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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