S100B Antibody (CL2720) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-43801
![S100B Antibody (CL2720) - Azide and BSA Free Immunohistochemistry-Paraffin: S100B Antibody [NBP3-43801]](https://resources.bio-techne.com/images/products/nbp3-43801_mouse-s100b-mab-cl2720-azide-and-bsa-free-28220251273746.jpg "Immunohistochemistry-Paraffin: S100B Antibody [NBP3-43801]")
![S100B Antibody (CL2720) - Azide and BSA Free Immunohistochemistry-Paraffin: S100B Antibody [NBP3-43801]](https://resources.bio-techne.com/images/products/nbp3-43801_mouse-s100b-mab-cl2720-azide-and-bsa-free-282202512295610.jpg "Immunohistochemistry-Paraffin: S100B Antibody [NBP3-43801]")
![S100B Antibody (CL2720) - Azide and BSA Free Western Blot: S100B Antibody [NBP3-43801]](https://resources.bio-techne.com/images/products/nbp3-43801_mouse-s100b-mab-cl2720-azide-and-bsa-free-28220251273728.jpg "Western Blot: S100B Antibody [NBP3-43801]")
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Validated by
Orthogonal Validation
Species Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # CL2720
Format
Azide and BSA Free
Concentration
LYOPH mg/ml
Product Specifications
Immunogen
This antibody was generated using a recombinant protein sequence of P04271, with the exact immunogen sequence remaining proprietary.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for S100B Antibody (CL2720) - Azide and BSA Free
Western Blot: S100B Antibody [NBP3-43801]
Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10Lane 2: Human Cerebral Cortex tissue
![S100B Antibody (CL2720) - Azide and BSA Free Immunohistochemistry-Paraffin: S100B Antibody [NBP3-43801]](https://resources.bio-techne.com/images/products/nbp3-43801_mouse-s100b-mab-cl2720-azide-and-bsa-free-282202512195454.jpg "Immunohistochemistry-Paraffin: S100B Antibody [NBP3-43801]")
Immunohistochemistry-Paraffin: S100B Antibody [NBP3-43801]
Staining of human cerebellum shows strong nuclear positivity in glial cells.![S100B Antibody (CL2720) - Azide and BSA Free Immunohistochemistry-Paraffin: S100B Antibody [NBP3-43801]](https://resources.bio-techne.com/images/products/nbp3-43801_mouse-s100b-mab-cl2720-azide-and-bsa-free-28220251235199.jpg "Immunohistochemistry-Paraffin: S100B Antibody [NBP3-43801]")
![S100B Antibody (CL2720) - Azide and BSA Free Immunohistochemistry-Paraffin: S100B Antibody [NBP3-43801]](https://resources.bio-techne.com/images/products/nbp3-43801_mouse-s100b-mab-cl2720-azide-and-bsa-free-28220251335866.jpg "Immunohistochemistry-Paraffin: S100B Antibody [NBP3-43801]")
![S100B Antibody (CL2720) - Azide and BSA Free Immunohistochemistry-Paraffin: S100B Antibody [NBP3-43801]](https://resources.bio-techne.com/images/products/nbp3-43801_mouse-s100b-mab-cl2720-azide-and-bsa-free-28220251324564.jpg "Immunohistochemistry-Paraffin: S100B Antibody [NBP3-43801]")
![S100B Antibody (CL2720) - Azide and BSA Free Immunohistochemistry-Paraffin: S100B Antibody [NBP3-43801]](https://resources.bio-techne.com/images/products/nbp3-43801_mouse-s100b-mab-cl2720-azide-and-bsa-free-28220251324587.jpg "Immunohistochemistry-Paraffin: S100B Antibody [NBP3-43801]")
Applications for S100B Antibody (CL2720) - Azide and BSA Free
Application
Recommended Usage
Immunohistochemistry-Paraffin
1:500 - 1:1000
Western Blot
1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Formulation, Preparation, and Storage
Purification
Protein A purified
Reconstitution
Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.
Formulation
Lyophilized from a 0.2 um filtered solution in PBS with Trehalose
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
LYOPH mg/ml
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: S100B
References
1. Yardan, T., Erenler, A. K., Baydin, A., Aydin, K., & Cokluk, C. (2011). Usefulness of S100B protein in neurological disorders. JPMA. The Journal of the Pakistan Medical Association, 61(3), 276-281.
2. Langeh, U., & Singh, S. (2021). Targeting S100B Protein as a Surrogate Biomarker and its Role in Various Neurological Disorders. Current neuropharmacology, 19(2), 265-277. https://doi.org/10.2174/1570159X18666200729100427
3. Thelin, E. P., Nelson, D. W., & Bellander, B. M. (2017). A review of the clinical utility of serum S100B protein levels in the assessment of traumatic brain injury. Acta neurochirurgica, 159(2), 209-225. https://doi.org/10.1007/s00701-016-3046-3
4. Wang, K. K., Yang, Z., Zhu, T., Shi, Y., Rubenstein, R., Tyndall, J. A., & Manley, G. T. (2018). An update on diagnostic and prognostic biomarkers for traumatic brain injury. Expert review of molecular diagnostics, 18(2), 165-180. https://doi.org/10.1080/14737159.2018.1428089
Long Name
S100 Calcium Binding Protein B
Alternate Names
beta (neural), NEF, S100, S100 beta, S100 calcium binding protein B, S100 calcium-binding protein B, S100 calcium-binding protein, beta (neural), S-100 calcium-binding protein, beta chain, 10protein S100-B, S-100 protein beta chain, S-100 protein subunit beta, S100beta
Gene Symbol
S100B
Additional S100B Products
Product Documents for S100B Antibody (CL2720) - Azide and BSA Free
Product Specific Notices for S100B Antibody (CL2720) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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