We would like to stain paraffin embedded human intestinal tissue for Calprotectin. One question will be which cells express the majority of Calprotectin - invading neutrophils vs epithelium for example. The antibody list you provide is quite extensive. Which antibody would you recommend for our purpose? Which tissue would you recommend as positive control.

Nine of our antibodies to Calprotectin have been validated for IHC-P with human tissue, and you can see all of these products. A number of our IHC images were generated using human spleen samples, and as such this may be a good choice of a positive control tissue. We do sell a human spleen slide product, which is suitable for IHC-P. Unfortunately I do not have an in-depth knowledge of Calprotectin, however the image shown for our antibody with catalogue number NBP1-02826 demonstrates clear staining of neutrophils. The following paper also suggests that the protein is abundant in neutrophils: PMID 11435495.

Have any of your S100A9 antibodies been tested for use in neutralization, or blocking assays, on human samples?

Unfortunately at this time we have not tested, or received customer feedback on our S100A9 antibodies for use in blocking or neutralizing assays. If you were planning on testing any of our S100A9 antibodies for neutralizing or blocking capabilities, we would recommend our Innovator’s Reward Program. Under the terms of this program we would be happy to provide a credit for a free vial in exchange for new data on this previously untested or reported application. Please submit this data in the form of an online review. Additional Innovators Reward Program information can be found using this link.

S100A9 Antibody [FITC]

Name: S100A9 Antibody [FITC]
Brand: Novus Biologicals
SKU: AF2065F

Novus Biologicals, part of Bio-Techne | Catalog # AF2065F

Submit a review

Product Datasheet / COA / SDS

View Available Conjugates

Conjugate

Catalog #

Unconjugated

AF2065

S100A9 Antibody [FITC]

Novus Biologicals, part of Bio-Techne | Catalog # AF2065F

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

Concentration

View all S100A9 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Applications for S100A9 Antibody [FITC]

Immunocytochemistry

Immunohistochemistry

Western Blot

Formulation, Preparation, and Storage

Purification

Formulation

Preservative

Concentration

Shipping

Stability & Storage

Background: S100A9

Long Name

Alternate Names

Gene Symbol

Additional S100A9 Products

Product Documents for S100A9 Antibody [FITC]

Product Datasheets

COA

Certificate of Analysis

SDS

Product Specific Notices for S100A9 Antibody [FITC]