S100A4 Antibody (CL0239) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-52891

Catalog #
Availability
Size / Price
Qty
NBP2-52891
NBP2-52891-25ul
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Conjugate
Catalog #
Alexa Fluor 350
NBP3-43988AF350
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Alexa Fluor 405
NBP3-43988AF405
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Alexa Fluor 488
NBP3-43988AF488
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Alexa Fluor 532
NBP3-43988AF532
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Alexa Fluor 594
NBP3-43988AF594
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Alexa Fluor 647
NBP3-43988AF647
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Alexa Fluor 700
NBP3-43988AF700
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Alexa Fluor 750
NBP3-43988AF750
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Alexa Fluor Plus 405
NBP3-43988AFP405
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Alexa Fluor Plus 488
NBP3-43988AFP488
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Alexa Fluor Plus 555
NBP3-43988AFP555
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Alexa Fluor Plus 594
NBP3-43988AFP594
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Alexa Fluor Plus 647
NBP3-43988AFP647
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Alexa Fluor Plus 680
NBP3-43988AFP680
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Allophycocyanin
NBP3-43988APC
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Biotin
NBP3-43988B
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CoraFluor 1
NBP3-43988CL1
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DyLight 350
NBP3-43988UV
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DyLight 405
NBP3-43988V
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DyLight 488
NBP3-43988G
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DyLight 550
NBP3-43988R
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DyLight 594
NBP3-43988DL594
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DyLight 650
NBP3-43988C
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DyLight 680
NBP3-43988FR
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DyLight 755
NBP3-43988IR
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FITC
NBP3-43988F
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HRP
NBP3-43988H
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Janelia Fluor 525
NBP3-43988JF525
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Janelia Fluor 549
NBP3-43988JF549
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Janelia Fluor 646
NBP3-43988JF646
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Janelia Fluor 669
NBP3-43988JF669
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mFluor Violet 450 SE
NBP3-43988MFV450
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mFluor Violet 500 SE
NBP3-43988MFV500
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mFluor Violet 610 SE
NBP3-43988MFV610
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PE
NBP3-43988PE
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PerCP
NBP3-43988PCP
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Key Product Details

Validated by

Knockout/Knockdown, Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # CL0239

Format

BSA Free

Concentration

1 mg/ml

View all S100A4 Antibodies »

Product Specifications

Immunogen

This antibody was developed using a recombinant protein derived from P26447, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for S100A4 Antibody (CL0239) - BSA Free

Western Blot: S100A4 Antibody (CL0239) [NBP2-52891]

Western Blot: S100A4 Antibody (CL0239) [NBP2-52891]

Western Blot: S100A4 Antibody (CL0239) [NBP2-52891] - Lane 1: Marker [kDa], Lane 2: Negative control (vector only transfected HEK293T lysate), Lane 3: S100A4 Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells)
Immunocytochemistry/ Immunofluorescence: S100A4 Antibody (CL0239) [NBP2-52891]

Immunocytochemistry/ Immunofluorescence: S100A4 Antibody (CL0239) [NBP2-52891]

Immunocytochemistry/Immunofluorescence: S100A4 Antibody (CL0239) [NBP2-52891] - Staining of BJ cells using the anti-S100A4 monoclonal antibody, showing specific staining in the plasma membrane in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).
Immunohistochemistry-Paraffin: S100A4 Antibody (CL0239) [NBP2-52891]

Immunohistochemistry-Paraffin: S100A4 Antibody (CL0239) [NBP2-52891]

Immunohistochemistry-Paraffin: S100A4 Antibody (CL0239) [NBP2-52891] - Staining in human tonsil and pancreas tissues. Corresponding S100A4 RNA-seq data are presented for the same tissues.
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Applications for S100A4 Antibody (CL0239) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry

1:2500 - 1:5000

Immunohistochemistry-Paraffin

1:2500 - 1:5000

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: S100A4

S100A4 is encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in motility, invasion, and tubulin polymerization. Chromosomal rearrangements and altered expression of this gene have been implicated in tumor metastasis. Multiple alternatively spliced variants, encoding the same protein, have been identified.

Long Name

S100 Calcium Binding Protein A4

Alternate Names

18A2, 42A, CAPL, MTS1, P9KA, PEL98

Gene Symbol

S100A4

Additional S100A4 Products

Product Documents for S100A4 Antibody (CL0239) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for S100A4 Antibody (CL0239) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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