RWDD3 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-76309

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NBP1-76309
NBP1-76309-0.025mg
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Conjugate
Catalog #
Alexa Fluor 350
NBP1-76309AF350
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Alexa Fluor 405
NBP1-76309AF405
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Alexa Fluor 488
NBP1-76309AF488
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Alexa Fluor 532
NBP1-76309AF532
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Alexa Fluor 594
NBP1-76309AF594
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Alexa Fluor 647
NBP1-76309AF647
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Alexa Fluor 700
NBP1-76309AF700
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Alexa Fluor 750
NBP1-76309AF750
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Alexa Fluor Plus 405
NBP1-76309AFP405
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Alexa Fluor Plus 488
NBP1-76309AFP488
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Alexa Fluor Plus 555
NBP1-76309AFP555
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Alexa Fluor Plus 594
NBP1-76309AFP594
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Alexa Fluor Plus 647
NBP1-76309AFP647
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Alexa Fluor Plus 680
NBP1-76309AFP680
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Allophycocyanin
NBP1-76309APC
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Biotin
NBP1-76309B
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CoraFluor 1
NBP1-76309CL1
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DyLight 350
NBP1-76309UV
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DyLight 405
NBP1-76309V
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DyLight 488
NBP1-76309G
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DyLight 550
NBP1-76309R
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DyLight 594
NBP1-76309DL594
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DyLight 650
NBP1-76309C
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DyLight 680
NBP1-76309FR
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DyLight 755
NBP1-76309IR
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FITC
NBP1-76309F
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HRP
NBP1-76309H
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Janelia Fluor 525
NBP1-76309JF525
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Janelia Fluor 549
NBP1-76309JF549
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Janelia Fluor 646
NBP1-76309JF646
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Janelia Fluor 669
NBP1-76309JF669
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mFluor Violet 450 SE
NBP1-76309MFV450
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mFluor Violet 500 SE
NBP1-76309MFV500
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mFluor Violet 610 SE
NBP1-76309MFV610
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PE
NBP1-76309PE
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PerCP
NBP1-76309PCP
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, ELISA

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all RWDD3 Antibodies »

Product Specifications

Immunogen

Antibody was raised against a 17 amino acid synthetic peptide from near the amino terminus of human RWDD3. The immunogen is located within the first 50 amino acids of RWDD3.

Reactivity Notes

Predicted species reactivity based on immunogen sequence: Bovine: (81%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for RWDD3 Antibody - BSA Free

Western Blot: RWDD3 AntibodyBSA Free [NBP1-76309]

Western Blot: RWDD3 AntibodyBSA Free [NBP1-76309]

Western Blot: RWDD3 Antibody [NBP1-76309] - Mouse kidney tissue lysate with RWDD3 antibody at 0.5 ug/mL in (A) the absence and (B) the presence of blocking peptide.

Applications for RWDD3 Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Western Blot

0.5 ug/ml

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: RWDD3

RWDD3 (RSUME), a small RWD-containing protein, has a central role in sumoylation by enhancing SUMO conjugation in the regulatory network of immune-inflammatory signals. RWDD3 increases Ikappabeta sumoylation and stability. In addition, RWDD3 inhibits TNF-alpha-induced kappaB-LUC (Luciferase) reporter activity, showing the functional consequence of Ikappabeta increased stability. RSUME-enhanced sumoylation of Ikappabeta leads to the inhibition of NF-kappabeta activity on two well-known inflammatory genes, IL-8 and cyclooxygenase-2 (Cox-2) and therefore may also favor anti-inflammatory pathways. Expression of RWDD3 was induced under hypoxic conditions and it has a potential role during vascularization. Both BMP-4 and RWDD3 may be interesting targets for inhibiting steps involved in pituitary tumorigenesis.

Alternate Names

DKFZP566K023, FLJ51225, RSUME, RWD domain containing 3, RWD domain-containing protein 3, RWD-containing sumoylation enhancer

Gene Symbol

RWDD3

UniProt

NP_056300 (Human)

Additional RWDD3 Products

Product Documents for RWDD3 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for RWDD3 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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