RUFY1 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-83606

Novus Biologicals, part of Bio-Techne
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NBP1-83606
NBP1-83606-25ul
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all RUFY1 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: DGLEKTNSKLQEELSAATDRICSLQEEQQQLREQNELIRERSEKSVEITKQDTKVELETYKQTRQGLDEMYSDVWKQLK

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit RUFY1 Antibody - BSA Free (NBP1-83606) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for RUFY1 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: RUFY1 Antibody [NBP1-83606]

Immunocytochemistry/ Immunofluorescence: RUFY1 Antibody [NBP1-83606]

Immunocytochemistry/Immunofluorescence: RUFY1 Antibody [NBP1-83606] - Staining of human cell line A-431 shows localization to nuclear speckles & cytosol.
Immunohistochemistry-Paraffin: RUFY1 Antibody [NBP1-83606]

Immunohistochemistry-Paraffin: RUFY1 Antibody [NBP1-83606]

Immunohistochemistry-Paraffin: RUFY1 Antibody [NBP1-83606] - Staining of human duodenum shows strong cytoplasmic positivity in glandular cells.
Western Blot: RUFY1 Antibody [NBP1-83606]

Western Blot: RUFY1 Antibody [NBP1-83606]

Western Blot: RUFY1 Antibody [NBP1-83606] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue. Lane 6: Human tonsil tissue
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Applications for RUFY1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: RUFY1

RUFY1 binds phospholipid vesicles containing phosphatidylinositol 3-phosphate and participates in early endosomaltrafficking

Alternate Names

La-binding protein 1, Rab4-interacting protein, RABIP4FYVE-finger protein EIP1, RUN and FYVE domain containing 1, RUN and FYVE domain-containing 1, RUN and FYVE domain-containing protein 1, ZFYVE12FLJ22251, Zinc finger FYVE domain-containing protein 12

Gene Symbol

RUFY1

Additional RUFY1 Products

Product Documents for RUFY1 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for RUFY1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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