RPGR Antibody - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-93653
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
Azide and BSA Free
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human RPGR (NP_001030025.1). MREPEELMPDSGAVFTFGKSKFAENNPGKFWFKNDVPVHLSCGDEHSAVVTGNNKLYMFGSNNWGQLGLGSKSAISKPTCVKALKPEKVKLAACGRNHTLVSTEGGNVYATGGNNEGQLGLGDTEERNTFHVISFFTSEHKIKQLSAGSNTSAALTEDGRLFMWGDNSEGQIGLKNVSNVCVPQQVTIGKPVSWISCGYYHSAFVTTDGELYVFGEPENGKLGLPNQLLGNHRTPQLVSEIPEKVIQVACGGEHTVVLTE
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
113 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Novus Biologicals Rabbit RPGR Antibody - Azide and BSA Free (NBP2-93653) is a polyclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for RPGR Antibody - Azide and BSA Free
![Western Blot: RPGR AntibodyAzide and BSA Free [NBP2-93653]](https://resources.bio-techne.com/images/products/RPGR-Antibody-Western-Blot-NBP2-93653-img0001.jpg "Western Blot: RPGR AntibodyAzide and BSA Free [NBP2-93653]")
Western Blot: RPGR AntibodyAzide and BSA Free [NBP2-93653]
Western Blot: RPGR Antibody [NBP2-93653] - Western blot analysis of extracts of Rat testis, using RPGR antibody (NBP2-93653) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.![Western Blot: RPGR AntibodyAzide and BSA Free [NBP2-93653]](https://resources.bio-techne.com/images/products/RPGR Antibody-Western Blot-NBP2-93653-img0002.jpg "Western Blot: RPGR AntibodyAzide and BSA Free [NBP2-93653]")
Western Blot: RPGR AntibodyAzide and BSA Free [NBP2-93653]
Western Blot: RPGR Antibody [NBP2-93653] - Western blot analysis of extracts of Mouse brain, using RPGR antibody (NBP2-93653) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.
Immunohistochemistry-paraffin-RPGR Antibody-NBP2-93653-
Immunohistochemistry-paraffin-RPGR Antibody-NBP2-93653-Analysis of paraffin-embedded mouse brain using RPGR Rabbit pAb at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Applications for RPGR Antibody - Azide and BSA Free
Application
Recommended Usage
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Immunocytochemistry/ Immunofluorescence
1:50 - 1:200
Immunohistochemistry
1:50 - 1:200
Western Blot
1:500 - 1:1000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol
Format
Azide and BSA Free
Preservative
0.05% Proclin 300
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: RPGR
Alternate Names
COD1, cone dystrophy 1 (X-linked), CORDX1RP3retinitis pigmentosa 15, CRD, orf15, PCDX, retinitis pigmentosa 3 GTPase regulator, retinitis pigmentosa GTPase regulator, RP15, X-linked retinitis pigmentosa GTPase regulator, XLRP3
Gene Symbol
RPGR
Additional RPGR Products
Product Documents for RPGR Antibody - Azide and BSA Free
Product Specific Notices for RPGR Antibody - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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