Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Human, Mouse

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.0 mg/ml

View all RPC62 Antibodies »

Product Specifications

Immunogen

The immunogen for this product maps to a region between residue 484 and 534 of human Polymerase (RNA) III (DNA directed) Polypeptide C (62kD) using the numbering given in entry NP_006459.3 (GeneID 10623).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for RPC62 Antibody - BSA Free

Western Blot: RPC62 Antibody [NBP1-71821]

Western Blot: RPC62 Antibody [NBP1-71821]

Western Blot: RPC62 Antibody [NBP1-71821] - Whole cell lysate from HeLa (H; 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (5, 15, and 50 mcg for WB) and mouse NIH3T3 (M; 50 mcg) cells. Affinity purified rabbit anti-RPC3 antibody used for WB at 0.4 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate. RPC3 was also immunoprecipitated by rabbit anti-RPC3 antibody NBP1-71820, which recognizes an upstream epitope.

Applications for RPC62 Antibody - BSA Free

Application
Recommended Usage

Western Blot

1:2000-1:10000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: RPC62

RPC62 may direct with other members of the subcomplex RNA Pol III binding to the TFIIIB-DNA complex via the interactions between TFIIIB and POLR3F. May be involved either in the recruitment and stablization of the subcomplex within RNA polymerase III, or in stimulating catalytic functions of other subunits during initiation.

Alternate Names

DNA-directed III 62 kDa polypeptide, DNA-directed RNA polymerase III subunit C, DNA-directed RNA polymerase III subunit RPC3, polymerase (RNA) III (DNA directed) polypeptide C (62kD), RNA polymerase III 62 kDa subunit, RNA polymerase III subunit C3, RPC62RPC3

Gene Symbol

POLR3C

UniProt

NP_006459.3 (Human)

Additional RPC62 Products

Product Documents for RPC62 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for RPC62 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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