Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Applications

Validated:

Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Simple Western, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.0 mg/ml

View all RPA2 Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 225 and the C-terminus (residue 270) of human Replication Protein A2 (32kD) using the numbering given in entry NP_002937.1 (GeneID 6118).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for RPA2 Antibody - BSA Free

Western Blot: RPA2 Antibody [NB100-332]

Western Blot: RPA2 Antibody [NB100-332]

Western Blot: RPA2 Antibody [NB100-332] - Detection of human and mouse RPA32 by western blot. Samples: Whole cell lysate (5 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RPA32 antibody NB100-332 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Immunohistochemistry-Paraffin: RPA2 Antibody [NB100-332]

Immunohistochemistry-Paraffin: RPA2 Antibody [NB100-332]

Immunohistochemistry-Paraffin: RPA2 Antibody [NB100-332] - Human breast carcinoma. Antibody: Affinity purified rabbit anti-RPA32 used at a dilution of 1:5,000 (0.2 ug/ml). Detection: DAB
Immunoprecipitation: RPA2 Antibody [NB100-332]

Immunoprecipitation: RPA2 Antibody [NB100-332]

Immunoprecipitation: RPA2 Antibody [NB100-332] - Detection of human RPA32 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells treated with 100 uM EPE for 4 hours (+) or mock treated (-). Antibodies: Affinity purified rabbit anti-RPA32 antibody NB100-332 used for IP at 3 ug per reaction. RPA32 was also immunoprecipitated by rabbit anti-Phospho RPA32 (S4/S8) recombinant monoclonal antibody [BL-165-5F1] (NB100-23017). For blotting immunoprecipitated Phospho RPA32 (S4/S8), NB100-23017 was used at 1:1000. Detection: Chemiluminescence with an exposure time time of 3 minutes.

Applications for RPA2 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:2000 - 1:10000

Immunohistochemistry-Paraffin

1:2000 - 1:10000

Immunoprecipitation

2 - 5 ug/mg lysate

Western Blot

1:2000-1:10000
Application Notes
Epitope retrieval with Tris-EDTA pH9.0 is recommended for FFPE tissue sections.
See Simple Western Antibody Database for Simple Western validation: tested in Human squamous carcinoma cells; antibody dilution of 1:500; separated by charge

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: RPA2

Replication protein A (RPA) is a single-stranded DNA binding protein. Human RPA is a heterotrimeric protein containing subunits of 70, 32 and 14 kDa. This protein complex is highly conserved in eukaryotes and is essential in DNA replication, homologous recombination and nucleotide excision repair. RPA has been shown to be a target for DNA-dependent protein kinase (DNA-PK) ataxia telangiectasia-mutated gene (ATM) and a cyclin dependent protein kinase. The middle subunit, RPA-32, is phosphorylated during the S-phase of the cell cycle, in response to DNA damage and during apoptotsis of Jurkat T- lymphocytes.

Long Name

Replication Protein A2, 32kDa

Alternate Names

REPA2, RPA32

Entrez Gene IDs

6118 (Human)

Gene Symbol

RPA2

UniProt

NP_002937.1 (Human)

Additional RPA2 Products

Product Documents for RPA2 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for RPA2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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