ROCK1 Antibody (24GB5310)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-50456

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NBP3-50456-100ul
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # 24GB5310

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all ROCK1 Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from human ROCK1 (Uniprot: Q13464)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ROCK1 Antibody (24GB5310)

ROCK1 Antibody (24GB5310)

Immunocytochemistry/ Immunofluorescence: ROCK1 Antibody (24GB5310)[NBP3-50456] -

Immunocytochemical staining of HeLa cells with ROCK1 antibody (1:1,000). Nuclei were stained blue with DAPI; ROCK1 was stained magenta with Alexa Fluor(R) 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 um.
ROCK1 Antibody (24GB5310)

Flow Cytometry: ROCK1 Antibody (24GB5310)[NBP3-50456] -

Flow cytometric analysis of ROCK1expression in HeLa cells using ROCK1 antibody (1:2,000). Green, isotype control; red, ROCK1.
ROCK1 Antibody (24GB5310)

Western Blot: ROCK1 Antibody (24GB5310)[NBP3-50456] -

Western blotting analysis using ROCK1 antibody (1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using NaQ(TM) ECL Substrate Kit.

Applications for ROCK1 Antibody (24GB5310)

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.4), 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: ROCK1

Rho-associated kinase I (ROCK1, ROK beta, p160ROCK) is a serine-threonine protein kinase and an effector of the small GTPase Rho. With N-terminus protein kinase domain and C-terminus Rho-binding domain/pleckstrin homology domain, ROCK1 can be activated by either RhoA or RhoB. In Rho specific ROCK1 activation, a Rho protein binds to the Rho-binding domain and induces a conformational change which opens the kinase domain for the phosphorylation of downstream effectors (1). Activated ROCK1 phosphorylates various signaling proteins, such as myosin light chain phosphatase, LIM kinases, and ezrin-radixin-moesin proteins. Caspase-3 also activates ROCK1; Caspase-3 cleaves ROCK1 at DETD1113/G sequence and removes its inhibitory c-terminal domain. This activation, independent of Rho activity, leads to apoptotic membrane blebbing (2). ROCK1 is involved in regulating actin cytoskeleton assembly, cell migration, centromere positing, smooth muscle contraction, and neurite outgrowth. Its involvement in tumor invasion, hypertension, and bronchial asthma makes ROCK1 an ideal target for drug development (3).

Long Name

Rho-associated, Coiled-Coil Containing Protein Kinase 1

Alternate Names

p160ROCK, ROK beta

Gene Symbol

ROCK1

Additional ROCK1 Products

Product Documents for ROCK1 Antibody (24GB5310)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for ROCK1 Antibody (24GB5310)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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