RNF20 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NB100-2242
![Western Blot: RNF20 Antibody [NB100-2242]](https://resources.bio-techne.com/images/products/RNF20-Antibody-Western-Blot-NB100-2242-img0006.jpg "Western Blot: RNF20 Antibody [NB100-2242]")
Conjugate
Catalog #
Key Product Details
Validated by
Independent Antibodies, Biological Validation
Species Reactivity
Validated:
Human, Mouse
Cited:
Human, Mouse
Applications
Validated:
Chromatin Immunoprecipitation (ChIP), Immunoprecipitation, Western Blot
Cited:
Chemotaxis, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
The immunogen recognized by this antibody maps to a region between residue 1 and 50 of human Ring Finger 20 using the numbering given in entry NP_062538.5 (GeneID 56254).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for RNF20 Antibody - BSA Free
Western Blot: RNF20 Antibody [NB100-2242]
Western Blot: RNF20 Antibody [NB100-2242] - Detection of Human and Mouse RNF20 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RNF20 antibody NB100-2242 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.![Western Blot: RNF20 Antibody [NB100-2242]](https://resources.bio-techne.com/images/products/RNF20-Antibody-Western-Blot-NB100-2242-img0002.jpg "Western Blot: RNF20 Antibody [NB100-2242]")
Western Blot: RNF20 Antibody [NB100-2242]
Western Blot: RNF20 Antibody [NB100-2242] - WB analysis of RNF20 in HeLa whole cell lysate. Image submitted by a verified customer review.![Immunoprecipitation: RNF20 Antibody [NB100-2242]](https://resources.bio-techne.com/images/products/RNF20-Antibody-Immunoprecipitation-NB100-2242-img0007.jpg "Immunoprecipitation: RNF20 Antibody [NB100-2242]")
Immunoprecipitation: RNF20 Antibody [NB100-2242]
Immunoprecipitation: RNF20 Antibody [NB100-2242] - Detection of human RNF20 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-RNF20 antibody NB100-2242 (lot NB100-2242-2) used for IP at 6 ug per reaction. RNF20 was also immunoprecipitated by a previous lot of this antibody (NB100-2242-1) and rabbit anti-RNF20 antibody NB100-2243. For blotting immunoprecipitated RNF20, NB100-2242 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.Applications for RNF20 Antibody - BSA Free
Application
Recommended Usage
Chromatin Immunoprecipitation (ChIP)
1:10-1:500
Immunoprecipitation
2-10 ug/mg lysate
Western Blot
1:2000-1:10000
Application Notes
Chip reactivity reported in (PMID: 21617703).
Reviewed Applications
Read 1 review rated 4 using NB100-2242 in the following applications:
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: RNF20
Alternate Names
BRE1, BRE1 E3 ubiquitin ligase homolog, BRE1-A, BRE1AMGC129667, E3 ubiquitin-protein ligase BRE1A, EC 6.3.2, EC 6.3.2.-, FLJ11189, FLJ20382, hBRE1MGC129668, homolog of S. cerevisiae BRE1, KAIA2779, ring finger protein 20KIAA2779
Entrez Gene IDs
56254 (Human)
Gene Symbol
RNF20
UniProt
Additional RNF20 Products
Product Documents for RNF20 Antibody - BSA Free
Product Specific Notices for RNF20 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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