RNF181 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-13240
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against a recombinant protein corresponding to the amino acids: ASYFDEHDCEPSDPEQETRTNMLLELARSLFNRMDFEDLGLVVDWDHHLPPPAAKTVVENLPRTVIRGSQAELKCP
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
Novus Biologicals Rabbit RNF181 Antibody - BSA Free (NBP2-13240) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for RNF181 Antibody - BSA Free
![Immunocytochemistry/ Immunofluorescence: RNF181 Antibody [NBP2-13240]](https://resources.bio-techne.com/images/products/RNF181-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-13240-img0005.jpg "Immunocytochemistry/ Immunofluorescence: RNF181 Antibody [NBP2-13240]")
Immunocytochemistry/ Immunofluorescence: RNF181 Antibody [NBP2-13240]
Immunocytochemistry/Immunofluorescence: RNF181 Antibody [NBP2-13240] - Staining of human cell line PC-3 shows localization to nucleoplasm & cytosol.![Immunohistochemistry-Paraffin: RNF181 Antibody [NBP2-13240]](https://resources.bio-techne.com/images/products/RNF181-Antibody-Immunohistochemistry-Paraffin-NBP2-13240-img0001.jpg "Immunohistochemistry-Paraffin: RNF181 Antibody [NBP2-13240]")
Immunohistochemistry-Paraffin: RNF181 Antibody [NBP2-13240]
Immunohistochemistry-Paraffin: RNF181 Antibody [NBP2-13240] - Staining of human liver shows moderate cytoplasmic positivity in hepatocytes.![RNF181 Antibody - BSA Free Western Blot: RNF181 Antibody - BSA Free [NBP2-13240]](https://resources.bio-techne.com/images/products/nbp2-13240_rabbit-polyclonal-rnf181-antibody-30520259595913.jpg "Western Blot: RNF181 Antibody - BSA Free [NBP2-13240]")
Western Blot: RNF181 Antibody - BSA Free [NBP2-13240]
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10Lane 2: Human cell line RT-4
Lane 3: Human cell line U-251MG sp
Applications for RNF181 Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:200 - 1:500
Immunohistochemistry-Paraffin
1:200 - 1:500
Western Blot
0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: RNF181
Alternate Names
E3 ubiquitin-protein ligase RNF181, EC 6.3.2.-, ring finger protein 181HSPC238
Gene Symbol
RNF181
Additional RNF181 Products
Product Documents for RNF181 Antibody - BSA Free
Product Specific Notices for RNF181 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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