RNF12 Antibody (1G10) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # H00051132-M01

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 1G10

Format

Azide and BSA Free

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all RNF12 Antibodies »

Product Specifications

Immunogen

RNF12 (NP_057204, 1 a.a. ~ 83 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MENSDSNDKGSGDQSAAQRRSQMDRLDREEAFYQFVNNLSEEDYRLMRDNNLLGTPGESTEEELLRRLQQIKEGPPPQNSDEN

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 27788132).

Localization

Cytoplasmic and Nuclear

Specificity

RNF12 - ring finger protein 12

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Novus Biologicals Mouse RNF12 Antibody (1G10) - Azide and BSA Free (H00051132-M01) is a monoclonal antibody validated for use in WB, ELISA, ICC/IF and IP. Anti-RNF12 Antibody: Cited in 7 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for RNF12 Antibody (1G10) - Azide and BSA Free

Western Blot: RNF12 Antibody (1G10) [H00051132-M01]

Western Blot: RNF12 Antibody (1G10) [H00051132-M01]

Western Blot: RNF12 Antibody (1G10) [H00051132-M01] - RNF12 monoclonal antibody (M01), clone 1G10. Analysis of RNF12 expression in Hela S3 NE.
Immunocytochemistry/ Immunofluorescence: RNF12 Antibody (1G10) [H00051132-M01]

Immunocytochemistry/ Immunofluorescence: RNF12 Antibody (1G10) [H00051132-M01]

Immunocytochemistry/Immunofluorescence: RNF12 Antibody (1G10) [H00051132-M01] - Analysis of monoclonal antibody to RNF12 on HeLa cell. Antibody concentration 30 ug/ml.
Western Blot: RNF12 Antibody (1G10) [H00051132-M01]

Western Blot: RNF12 Antibody (1G10) [H00051132-M01]

Western Blot: RNF12 Antibody (1G10) [H00051132-M01] - Analysis of RNF12 expression in transfected 293T cell line by RNF12 monoclonal antibody (M01), clone 1G10.Lane 1: RNF12 transfected lysate (Predicted MW: 68.5 KDa).Lane 2: Non-transfected lysate.
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Applications for RNF12 Antibody (1G10) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: RNF12

The protein encoded by this gene is a RING-H2 zinc finger protein. It has been shown to be a ubiquitin protein ligase that targets LIM domain binding 1 (LDB1/CLIM), and causes proteasome-dependent degradation of LDB1. This protein and LDB1 are co-repressors of LHX1/LIM-1, a homeodomain transcription factor. Alternatively spliced transcript variants encoding the same protein have been reported.

Alternate Names

DKFZp686N06224, E3 ubiquitin-protein ligase RLIM, E3 ubiquitin-protein ligase RNF12, EC 6.3.2, EC 6.3.2.-, FLJ25923, FLJ41093, LIM domain-interacting RING finger protein, MGC15161, NY-REN-43, Renal carcinoma antigen NY-REN-43, RING finger LIM domain-binding protein, RING finger protein 12FLJ42887, ring finger protein, LIM domain interacting, ring zinc finger LIM domain binding protein, ring zinc finger protein NY-REN-43antigen, R-LIM, RNF12FLJ45986

Entrez Gene IDs

51132 (Human)

Gene Symbol

RLIM

OMIM

300379 (Human)

UniProt

NP_057204 (Human)

Additional RNF12 Products

Product Documents for RNF12 Antibody (1G10) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for RNF12 Antibody (1G10) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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