RNA Polymerase II/POLR2A Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-55663
Key Product Details
Species Reactivity
Validated:
Human
Predicted:
Mouse (100%), Rat (100%). Backed by our 100% Guarantee.
Applications
Chromatin Immunoprecipitation-exo-Seq, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against a recombinant protein corresponding to the following amino acid sequence: GFGDDLNCIFNDDNAEKLVLRIRIMNSDENKMQEEEEVVDKMDDDVFLRCIESNMLTDMTLQGIEQISKVYMHLPQTDNKKKIIITEDGEFKALQEWILETDGVSLMRVLS
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for RNA Polymerase II/POLR2A Antibody - BSA Free
![Immunocytochemistry/ Immunofluorescence: RNA Polymerase II/POLR2A Antibody [NBP2-55663]](https://resources.bio-techne.com/images/products/RNA-Polymerase-II-POLR2A-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-55663-img0001.jpg "Immunocytochemistry/ Immunofluorescence: RNA Polymerase II/POLR2A Antibody [NBP2-55663]")
Immunocytochemistry/ Immunofluorescence: RNA Polymerase II/POLR2A Antibody [NBP2-55663]
Immunocytochemistry/Immunofluorescence: RNA Polymerase II/POLR2A Antibody [NBP2-55663] - Staining of human cell line U-2 OS shows localization to nucleoplasm. Antibody staining is shown in green.![RNA Polymerase II/POLR2A Antibody - BSA Free Chromatin Immunoprecipitation-exo-Seq: RNA Polymerase II/POLR2A Antibody - BSA Free [NBP2-55663]](https://resources.bio-techne.com/images/products/nbp2-55663_rabbit-polyclonal-rna-polymerase-ii-polr2a-antibody-256202599323.jpg "Chromatin Immunoprecipitation-exo-Seq: RNA Polymerase II/POLR2A Antibody - BSA Free [NBP2-55663]")
Chromatin Immunoprecipitation-exo-Seq: RNA Polymerase II/POLR2A Antibody - BSA Free [NBP2-55663]
ChIP-Exo-Seq composite graph for Anti-POLR2A (NBP2-55663) tested in K562 cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.Applications for RNA Polymerase II/POLR2A Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Application Notes
Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: RNA Polymerase II/POLR2A
Long Name
DNA-directed RNA Polymerase II Subunit RPB1
Alternate Names
POLR2, POLR2A, POLRA, RPB1, RPO2, RPOL2
Gene Symbol
POLR2A
Additional RNA Polymerase II/POLR2A Products
Product Documents for RNA Polymerase II/POLR2A Antibody - BSA Free
Product Specific Notices for RNA Polymerase II/POLR2A Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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