RNA Helicase A Antibody (2I6C5)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16435
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 2I6C5
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1100-1200 of human RNA Helicase A (Q08211). ITGLRAAMEALVVEVTKQPAIISQLDPVNERMLNMIRQISRPSAAGINLMIGSTRYGDGPRPPKMARYDNGSGYRRGGSSYSGGGYGGGYSSGGYGSGGYG
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for RNA Helicase A Antibody (2I6C5)
![Western Blot: RNA Helicase A Antibody (2I6C5) [NBP3-16435]](https://resources.bio-techne.com/images/products/RNA-Helicase-A-Antibody-2I6C5-Western-Blot-NBP3-16435-img0006.jpg "Western Blot: RNA Helicase A Antibody (2I6C5) [NBP3-16435]")
Western Blot: RNA Helicase A Antibody (2I6C5) [NBP3-16435]
Western Blot: RNA Helicase A Antibody (2I6C5) [NBP3-16435] - Western blot analysis of extracts of various cell lines, using DHX9/RNA Helicase A Rabbit mAb (NBP3-16435) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.![Immunoprecipitation: RNA Helicase A Antibody (2I6C5) [NBP3-16435]](https://resources.bio-techne.com/images/products/RNA-Helicase-A-Antibody-2I6C5-Immunoprecipitation-NBP3-16435-img0007.jpg "Immunoprecipitation: RNA Helicase A Antibody (2I6C5) [NBP3-16435]")
Immunoprecipitation: RNA Helicase A Antibody (2I6C5) [NBP3-16435]
Immunoprecipitation: RNA Helicase A Antibody (2I6C5) [NBP3-16435] - Immunoprecipitation analysis of 300ug extracts of HeLa cells using 3ug DHX9/RNA Helicase A antibody (NBP3-16435). Western blot was performed from the immunoprecipitate using DHX9/RNA Helicase A antibody (NBP3-16435) at a dilition of 1:2000.
Immunohistochemistry: RNA Helicase A Antibody (2I6C5) [NBP3-16435] -
Immunohistochemistry: RNA Helicase A Antibody (2I6C5) [NBP3-16435] - Immunohistochemistry analysis of paraffin-embedded Rat brain using RNA Helicase A Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
Applications for RNA Helicase A Antibody (2I6C5)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50 - 1:200
Immunohistochemistry
1:50 - 1:200
Immunoprecipitation
1:1000 - 1:5000
Western Blot
1:500 - 1:2000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol, 0.05% BSA
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: RNA Helicase A
Long Name
ATP-dependent RNA helicase A
Alternate Names
DDX9, DEAH box protein 9, DExH-box helicase 9, DHX9, EC 3.6.4.13, Leukophysin, LKP, NDH II, NDH2
Gene Symbol
DHX9
Additional RNA Helicase A Products
Product Documents for RNA Helicase A Antibody (2I6C5)
Product Specific Notices for RNA Helicase A Antibody (2I6C5)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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