RKIP/PBP Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-53808

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NB100-53808
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (100%), Canine (100%), Mouse (100%), Porcine (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all RKIP Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-DPDAPSRKDPKYRE corresponding to internal region according to NP_002558.1.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for RKIP/PBP Antibody

Western Blot: RKIP/PBP Antibody [NB100-53808]

Western Blot: RKIP/PBP Antibody [NB100-53808]

Western Blot: RKIP/PBP Antibody [NB100-53808] - Staining of human prostate lysate (35 ug protein in RIPA buffer). Antibody at 0.01 ug/mL. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: RKIP/PBP Antibody [NB100-53808]

Immunohistochemistry-Paraffin: RKIP/PBP Antibody [NB100-53808]

Immunohistochemistry-Paraffin: RKIP/PBP Antibody [NB100-53808] - Staining of paraffin embedded human kidney. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Immunohistochemistry-Paraffin: RKIP/PBP Antibody [NB100-53808]

Immunohistochemistry-Paraffin: RKIP/PBP Antibody [NB100-53808]

Immunohistochemistry-Paraffin: RKIP/PBP Antibody [NB100-53808] - Staining of paraffin embedded human adrenal gland. Antibody at 2.5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Applications for RKIP/PBP Antibody

Application
Recommended Usage

Immunohistochemistry

5 ug/mL

Immunohistochemistry-Paraffin

5 ug/mL

Peptide ELISA

Detection limit 1:128000

Western Blot

0.01 - 0.03 ug/mL
Application Notes
WB: Approx. 21 kDa band observed in human prostate lysates (calculated MW of 21.1 kDa band according to NP_002558.1).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: RKIP

PBP, also known as RKIP (RAF kinase inhibitor protein), binds ATP, opioids and phosphatidylethanolamine. PBP is able to regulate Aurora kinase B expression and the spindle checkpoint through interaction with the RAF1/MEK/ERK cascade. PBP is able to bind to RAF1 and MEK and competitively disrupt the interaction between these proteins. PBP may be involved in the function of the presynaptic cholinergic neurons of the central nervous system. It increases the production of choline acetyltransferase but not acetylcholinesterase. PBP has been implicated as a suppressor of metastasis.

Long Name

Raf kinase inhibitory protein

Alternate Names

HCNPpp, Neuropolypeptide h3, PBP, PEBP-1, PEBP1

Entrez Gene IDs

5037 (Human); 23980 (Mouse); 29542 (Rat)

Gene Symbol

PEBP1

UniProt

NP_002558.1 (Human)

Additional RKIP Products

Product Documents for RKIP/PBP Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for RKIP/PBP Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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