RKIP/PBP Antibody (24GB860)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-51461

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NBP3-51461-100ul
NBP3-51461-20ul
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # 24GB860

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all RKIP Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from human RKIP/PBP (Uniprot: P30086)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for RKIP/PBP Antibody (24GB860)

RKIP/PBP Antibody (24GB860)

Immunocytochemistry/ Immunofluorescence: RKIP/PBP Antibody (24GB860)[NBP3-51461] -

Immunocytochemical staining of Hela cells with RKIP/PBP antibody (1:1,000). Nuclei were stained blue with DAPI; RKIP/PBP was stained magenta with Alexa Fluor(R) 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 um.
RKIP/PBP Antibody (24GB860)

Flow Cytometry: RKIP/PBP Antibody (24GB860)[NBP3-51461] -

Validation of RKIP/PBP knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with RKIP/PBP antibody (1:2,000) and analyzed using BD flow cytometer.
RKIP/PBP Antibody (24GB860)

Flow Cytometry: RKIP/PBP Antibody (24GB860)[NBP3-51461] -

Flow cytometric analysis of RKIP/PBPexpression in HeLa cells using RKIP/PBP antibody (1:2,000). Green, isotype control; red, PEBP1.

Applications for RKIP/PBP Antibody (24GB860)

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Knockdown Validated

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.4), 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: RKIP

PBP, also known as RKIP (RAF kinase inhibitor protein), binds ATP, opioids and phosphatidylethanolamine. PBP is able to regulate Aurora kinase B expression and the spindle checkpoint through interaction with the RAF1/MEK/ERK cascade. PBP is able to bind to RAF1 and MEK and competitively disrupt the interaction between these proteins. PBP may be involved in the function of the presynaptic cholinergic neurons of the central nervous system. It increases the production of choline acetyltransferase but not acetylcholinesterase. PBP has been implicated as a suppressor of metastasis.

Long Name

Raf kinase inhibitory protein

Alternate Names

HCNPpp, Neuropolypeptide h3, PBP, PEBP-1, PEBP1

Gene Symbol

PEBP1

Additional RKIP Products

Product Documents for RKIP/PBP Antibody (24GB860)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for RKIP/PBP Antibody (24GB860)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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