RIOK1 Antibody

Western Blot: RIOK1 Antibody [NBP1-30103]

Western Blot: RIOK1 Antibody [NBP1-30103] - Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IPloaded) and HEK293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-RIOK1 antibody used for WB at 0.04 ug/ml (A) and 0.4 ug/ml (B) and used for IP at 3 ug/mg lysate. RIOK1 was also immunoprecipitated by rabbit anti-RIOK1 antibody, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).

Western Blot: RIOK1 Antibody [NBP1-30103] -

(A) FP results for linear sequence 29 and2nd and3rd generation macrocyclic PAPIIs with PRMT5-MEP50 (n = 3). (B) FP competition of representative linear and macrocyclicpeptides with FITC-labeled 50 for binding to PRMT5-MEP50(n = 3). (C) FP competition of 2nd and 3rd generationPAPIIs and the unlabeled pICln protein with the Alexa 488-labeledfull-length human pICln protein for binding to PRMT5-MEP50 (n = 3). (D) Comparison of the effects of linear and cyclicpeptides on the melting temperature of the TIM-MEP50 complex, as determinedby TSA (n = 6). (E) Pull-down assay with peptides 55 and scrambled 56 immobilized on the DBCO beadsusing MCF7 cell lysate. Immobilized 55 was also testedwith the lysate containing 100 μM of free 56. (F)GFP-IP from the lysate of Flp-In T-REx 293-GFP and Flp-In T-REx 293-GFP-PRMT5overexpressing cells, testing compound 53 and scrambled 54 at 50 μM. (G) GFP-IP from Flp-In T-REx 293-GFP-pIClnand Flp-In T-Rex 293-GFP-RioK1 cytoplasmic extract, testing 53 and 54 at 50 μM. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36378254), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: RIOK1 Antibody [NBP1-30103] -

(A) FP results for linear sequence 29 and2nd and3rd generation macrocyclic PAPIIs with PRMT5-MEP50 (n = 3). (B) FP competition of representative linear and macrocyclicpeptides with FITC-labeled 50 for binding to PRMT5-MEP50(n = 3). (C) FP competition of 2nd and 3rd generationPAPIIs and the unlabeled pICln protein with the Alexa 488-labeledfull-length human pICln protein for binding to PRMT5-MEP50 (n = 3). (D) Comparison of the effects of linear and cyclicpeptides on the melting temperature of the TIM-MEP50 complex, as determinedby TSA (n = 6). (E) Pull-down assay with peptides 55 and scrambled 56 immobilized on the DBCO beadsusing MCF7 cell lysate. Immobilized 55 was also testedwith the lysate containing 100 μM of free 56. (F)GFP-IP from the lysate of Flp-In T-REx 293-GFP and Flp-In T-REx 293-GFP-PRMT5overexpressing cells, testing compound 53 and scrambled 54 at 50 μM. (G) GFP-IP from Flp-In T-REx 293-GFP-pIClnand Flp-In T-Rex 293-GFP-RioK1 cytoplasmic extract, testing 53 and 54 at 50 μM. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36378254), licensed under a CC-BY license. Not internally tested by Novus Biologicals.