HEK293T cells were transfected with the pCMV6-ENTRY control ( Left lane) or pCMV6-ENTRY RHOH ( Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RHOH (1:2000). Positive lysates

Applications for RhoH Antibody (OTI9F4)

Application

Recommended Usage

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

>95%

Reconstitution

For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized powder ( 1X PBS, pH 7.3,)

Preservative

8% Trehalose

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 degrees C. Avoid freeze/thaw cycles.

Background: RhoH

The Rho subfamily of small GTP-binding proteins mediates many fundamental cellular functions. The commonly studied members (Rho, Rac, and CDC42) regulate actin reorganization and affect diverse cellular responses, including adhesion, cytokinesis, and motility. RhoH, also known as TTF (Translocation Three Four), Rho-related GTP-binding protein and ras homolog gene family member H, is unlike most other small G proteins. Most small G proteins are expressed ubiquitously, however, Rho H is expressed only in hemopoietic cells and tissues. Translocations and a high frequency of Rho H mutation have been detected in primary lymphoma cells. Rho H expression has also been observed in activated neutrophils. RhoH is GTPase deficient, remaining in a GTP-bound activated state without cycling. Rho H may be involved in the functional differentiation of T cells and in cytoskeleton organization. The RhoH/TTF (ARHH) gene maps to chromosome 4p13 and encodes a 191 -amino acid polypeptide.

Alternate Names

ras homolog gene family, member H, translocation three four

Gene Symbol

RHOH

Additional RhoH Products

Product Documents for RhoH Antibody (OTI9F4)

Product Datasheets

Download Product Datasheets

COA

Product Specific Notices for RhoH Antibody (OTI9F4)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations
Reviews

RhoH Antibody (OTI9F4)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-44854

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

Concentration

View all RhoH Antibodies »

Product Specifications

Immunogen

Clonality

Host

Isotype

Scientific Data Images for RhoH Antibody (OTI9F4)

Western Blot;RhoH Antibody (OTI9F4)[NBP3-44854]-

Applications for RhoH Antibody (OTI9F4)

Western Blot

Formulation, Preparation, and Storage

Purification

Reconstitution

Formulation

Preservative

Concentration

Shipping

Stability & Storage

Background: RhoH

Alternate Names

Gene Symbol

Additional RhoH Products

Product Documents for RhoH Antibody (OTI9F4)

Product Datasheets

COA

Certificate of Analysis

Product Specific Notices for RhoH Antibody (OTI9F4)

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