RelA/NFkB p65 Antibody (23GB1490)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-51672

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NBP3-51672-100ul
NBP3-51672-20ul
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # 23GB1490

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all RelA/NFkB p65 Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from human RelA/NFkB p65 (Uniprot: Q04206)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for RelA/NFkB p65 Antibody (23GB1490)

RelA/NFkB p65 Antibody (23GB1490)

Immunocytochemistry/ Immunofluorescence: RelA/NFkB p65 Antibody (23GB1490)[NBP3-51672] -

Immunocytochemical staining of HepG2 cells with RelA/NFkB p65 antibody (1:1,000). Nuclei were stained blue with DAPI; RelA/NFkB p65 was stained magenta with Alexa Fluor(R) 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 um.
RelA/NFkB p65 Antibody (23GB1490)

Flow Cytometry: RelA/NFkB p65 Antibody (23GB1490)[NBP3-51672] -

Flow cytometric analysis of RelA/NFkB p65expression in HepG2 cells using RelA/NFkB p65 antibody (1:2,000). Green, isotype control; red, NF-kB p65.
RelA/NFkB p65 Antibody (23GB1490)

Western Blot: RelA/NFkB p65 Antibody (23GB1490)[NBP3-51672] -

Western blotting analysis using RelA/NFkB p65 antibody (1:10,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using FeQ(TM) ECL Substrate Kit.

Applications for RelA/NFkB p65 Antibody (23GB1490)

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Knockdown Validated

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.4), 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: RelA/NFkB p65

Nuclear factor B (NF-B) encompasses an important family of inducible transcriptional activators that regulate a wide variety of cellular and viral genes. The family members include p50, p52, p65 (RelA), c-Rel, and RelB. The Nf-kB p65 subunit, similar to two others in the B family, RelB and c-Rel, contains two transactivation domains in the C-terminal region of the protein. Association with inhibitory proteins of the I B family, retains NF- B in the cytoplasm. Degradation of IB proteins exposes the nuclear localization sequence (NLS), leading to nuclear translocation and subsequent binding of NF-B to DNA. In addition to the nuclear translocation and DNA binding, Nf-kB's transcriptional activity is also regulated by coactivators and corepressors in the nucleus. Interaction of p65 with coactivators; CREB-binding protein and p300, increases its transcriptional activity.

Long Name

v-rel Reticuloendotheliosis Viral Oncogene Homolog A

Alternate Names

NFkB p65, NFKB3, p65RelA

Gene Symbol

RELA

Additional RelA/NFkB p65 Products

Product Documents for RelA/NFkB p65 Antibody (23GB1490)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for RelA/NFkB p65 Antibody (23GB1490)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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