Reg3B Antibody [CoraFluor™ 1]
Novus Biologicals, part of Bio-Techne | Catalog # AF5110CL1
![Reg3B Antibody [CoraFluor™ 1]](https://resources.bio-techne.com/images/products/af5110cl1_sheep-reg3b-pab-corafluor-1-10920251472921.png "Product Feature: CoraFluor Probes for TR-FRET")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Mouse
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Flow Cytometry
Label
CoraFluor 1
Antibody Source
Polyclonal Sheep IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
E. coli-derived recombinant mouse Reg3B
Glu27-Gly175
Accession # P35230
Glu27-Gly175
Accession # P35230
Specificity
Detects mouse Reg3B in direct ELISAs and Western blots. In direct ELISAs, approximately 75% cross-reactivity with recombinant rat (rr) Reg3B is observed. In Western blots approximately 30% cross-reactivity with rrReg3B is observed. In direct ELISAs and Western blots, less than 1% cross‑reactivity with rrReg3A, recombinant mouse (rm) Reg3A, and rmReg3G is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Description
This conjugate is made on demand and is not held in inventory. Each order represents a unique lot. All conjugates are generated using validated conjugation protocols and meet strict degree of labeling (F/P) specifications; additional information is available upon request. The antibody concentration is provided on the individual product label.
For specialized conjugation needs, like large quantities, specific concentrations, and defined F/P ratios, bulk and custom antibody conjugation services are available.
For specialized conjugation needs, like large quantities, specific concentrations, and defined F/P ratios, bulk and custom antibody conjugation services are available.
Scientific Data Images for Reg3B Antibody [CoraFluor™ 1]
Product Feature: CoraFluor Probes for TR-FRET
CoraFluor™ 1, amine reactive (Catalog:7920) and CoraFluor™ 2, amine reactive (Catalog # 7950) are terbium-based probes that have been developed for use as TR-FRET donors. They emit wavelengths compatible with commonly used fluorescent acceptor dyes such as BODIPY® (or BDY) and Janelia Fluor® dyes, FITC (Catalog # 5440), TMR and Cyanine 5 (Catalog # 5436). CoraFluor™ fluorescence is brighter and more stable in biological media than existing TR-FRET donors, leading to enhanced sensitivity and improved data generation. CoraFluor™ 1 exhibits excitation upon exposure to a 337 nm UV laser.Applications for Reg3B Antibody [CoraFluor™ 1]
Application
Recommended Usage
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Frozen
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Paraffin
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Recommended applications are based on validated applications from the unconjugated base product AF5110. This conjugated antibody is not kept in inventory and is made to order using validated conjugation protocols.
Spectra Viewer
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
Spectra Viewer
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark. Do not freeze.
Background: Reg3B
Reg3B (regenerating islet-derived protein III beta; also PAP-I and HIP) is a secreted 17 kDa member of the Reg gene family, C-type lectin gene superfamily. It is found in pancreatic acinar cells, intestinal enterocytes, and developing spinal motoneurons. Reg3B may play a role in intestinal epithelial cell renewal. Mouse Reg3B contains one C-type lectin domain (aa 38 - 173). Mature mouse Reg3B is 59%, 71% and 51% aa identical to the products of three other Reg3 named genes termed Reg III alpha, gamma and delta, respectively. Mature mouse Reg3B is also 91% and 67% aa identical to rat and human Reg3B, respectively.
Long Name
Regenerating Islet-derived IIIb
Alternate Names
PAP1
Gene Symbol
REG3A
Additional Reg3B Products
Product Documents for Reg3B Antibody [CoraFluor™ 1]
Product Specific Notices for Reg3B Antibody [CoraFluor™ 1]
Sold for research purposes only under agreement from Massachusetts General Hospital, US Patent No. 12,134,721. A license is required for development of new commercial assay platforms (e.g., microarrays, microfluidics), diagnostic use or other unlicensed uses. Please contact Technical Support for further details: techsupport@bio-techne.com.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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