RbBP5 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NB600-252
![Western Blot: RbBP5 Antibody [NB600-252]](https://resources.bio-techne.com/images/products/RbBP5-Antibody-Western-Blot-NB600-252-img0011.jpg "Western Blot: RbBP5 Antibody [NB600-252]")
![Western Blot: RbBP5 Antibody [NB600-252]](https://resources.bio-techne.com/images/products/RbBP5-Antibody-Western-Blot-NB600-252-img0012.jpg "Western Blot: RbBP5 Antibody [NB600-252]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Human
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Chromatin Immunoprecipitation (ChIP), Chromatin Immunoprecipitation Sequencing
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
The immunogen recognized by this antibody maps to a region between residue 500 and the C-terminus (residue 538) of human retinoblastoma binding protein 5 using the numbering given in entry NP_005048.2 (GeneID 5929).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
Novus Biologicals Rabbit RbBP5 Antibody - BSA Free (NB600-252) is a polyclonal antibody validated for use in IHC, WB, ICC/IF, IP and ChIP. Anti-RbBP5 Antibody: Cited in 13 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for RbBP5 Antibody - BSA Free
Western Blot: RbBP5 Antibody [NB600-252]
Western Blot: RbBP5 Antibody [NB600-252] - Whole cell lysate (50 ug) from TCMK-1 and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RbBP5 antibody used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.![Immunohistochemistry-Paraffin: RbBP5 Antibody [NB600-252]](https://resources.bio-techne.com/images/products/RbBP5-Antibody-Immunohistochemistry-NB600-252-img0008.jpg "Immunohistochemistry-Paraffin: RbBP5 Antibody [NB600-252]")
Immunohistochemistry-Paraffin: RbBP5 Antibody [NB600-252]
Immunohistochemistry-Paraffin: RbBP5 Antibody [NB600-252] - Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti- RbBP5 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Chromatin Immunoprecipitation Sequencing: RbBP5 Antibody [NB600-252] - Localization of RbBP5 Binding Sites by ChIP-sequencing. Chromatin from K562 cells was immunoprecipitated with anti-RbBP5 antibody NB600-252 and analyzed by DNA sequencing. The figure illustrates the peak distribution of RbBP5 binding within a 500 Kb region of chromosome 1 as detected using anti-RbBP5 antibody NB600-252. ChIP-seq validation performed by Diogenode, Denville, NJ.
Applications for RbBP5 Antibody - BSA Free
Application
Recommended Usage
Chromatin Immunoprecipitation (ChIP)
10 ug
Chromatin Immunoprecipitation Sequencing
2 ug
Immunocytochemistry/ Immunofluorescence
1-4 ug/ml
Immunohistochemistry
1:500- 1:2000
Immunohistochemistry-Paraffin
1:500- 1:2000
Immunoprecipitation
2-10 ug/mg lysate
Western Blot
1:10000 - 1:25000
Application Notes
Reported use in ChIP assays (see Dou et al). Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Reviewed Applications
Read 1 review rated 5 using NB600-252 in the following applications:
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: RbBP5
Alternate Names
RBQ3RBBP-5, retinoblastoma binding protein 5, Retinoblastoma-binding protein RBQ-3, Set1c WD40 repeat protein, homolog, SWD1
Gene Symbol
RBBP5
UniProt
Additional RbBP5 Products
Product Documents for RbBP5 Antibody - BSA Free
Product Specific Notices for RbBP5 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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