Detects rat TSH beta in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody shows approximately 5% cross‑reactivity with recombinant human TSH beta.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG_2B

Applications for Rat TSH beta Antibody

Application

Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Rat TSH beta

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS and NaCl with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TSH beta

TSH beta is the 138 amino acid (aa), 15 kDa unique subunit of Thyroid Stimulating Hormone (TSH), which shares a common alpha subunit with other glycoprotein hormones lutropin (LH), follitropin (FSH) and chorionic gonadotropin (CG). Production of TSH by the anterior pituitary gland is stimulated by the hypothalamic peptide TRH, and inhibited by feedback from the thyroid hormones triiodothyronine (T3) and thyroxine (T4). Mature rat TSH beta shares 92% and 90% aa sequence identity with mouse and human TSH beta, respectively.

Long Name

Thyroid-stimulating Hormone Subunit beta

Alternate Names

Thyrotropin beta, TSHB

Entrez Gene IDs

7252 (Human); 22094 (Mouse); 25653 (Rat)

Gene Symbol

TSHB

UniProt

P04652

Additional TSH beta Products

Product Documents for Rat TSH beta Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Rat TSH beta Antibody

For research use only

Citations
Reviews

Rat TSH beta Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB4507

Key Product Details

Species Reactivity

Validated:

Cited:

Applications