Rat RAGE Antibody

Name: Rat RAGE Antibody
Brand: R&D Systems
SKU: AF1616
Rating: 5 (1 reviews)

R&D Systems, Inc. a Bio-Techne Brand

Catalog # AF1616

Citations (2)

(1)

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Key Product Details

Species Reactivity:	Rat
Immunogen:	Mouse myeloma cell line NS0-derived recombinant rat RAGE Gln24-Ala342 Accession # Q63495
Applications:	Block/Neutralize, ELISA Capture, Western Blot
Label:	Unconjugated
Source:	Polyclonal Goat IgG
Specificity:	Detects rat RAGE in ELISAs and Western blots. In sandwich immunoassays, less than 0.1% cross-reactivity with recombinant human RAGE and recombinant canine RAGE is observed.

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Applications for Rat RAGE Antibody

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	Recombinant Rat RAGE Fc Chimera (Catalog # 1616-RG)
Blockade of Receptor-ligand Interaction	In a functional ELISA, 5-25 µg/mL of this antibody will block 50% of the binding of 5 μg/mL of biotinylated AGE-BSA to immobilized Recombinant Rat RAGE Fc Chimera (Catalog # 1616-RG) coated at 5 µg/mL (100 µL/well). At 166 μg/mL, this antibody will block >90% of the binding.

Rat RAGE Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Capture (Matched Antibody Pair)

0.2-0.8 µg/mL

Use in combination with:

Detection Reagent: Rat RAGE Biotinylated Antibody (Catalog # BAF1616)

Standard: Recombinant Rat RAGE Fc Chimera Protein, CF (Catalog # 1616-RG)

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Formulation, Preparation and Storage

Purification	Antigen Affinity-purified
Endotoxin level	<0.10 EU per 1 μg of the antibody by the LAL method.
Reconstitution	Reconstitute at 0.2 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

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The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: RAGE/AGER

Advanced glycation endproducts (AGE) are adducts formed by the non-enzymatic glycation or oxidation of macromolecules (1). AGE forms during aging and its formation is accelerated under pathophysiologic states such as diabetes, Alzheimer’s disease, renal failure and immune/inflammatory disorders. Receptor for Advanced Glycation Endoproducts (RAGE), named for its ability to bind AGE, is a multiligand receptor belonging to the immunoglobulin (Ig) superfamily. Besides AGE, RAGE binds amyloid beta -peptide, S100/calgranulin family proteins, high mobility group B1 (HMGB1, also know as amphoterin) and leukocyte integrins (1, 2).

The human RAGE gene encodes a 404 amino acid (aa) residues type I transmembrane glycoprotein with a 22 aa signal peptide, a 319 aa extracellular domain containing an Ig-like V-type domain and two Ig-like Ce-type domains, a 21 aa transmembrane domain and a 40 aa cytoplasmic domain (3). The V-type domain and the cytoplasmic domain are important for ligand binding and for intracellular signaling, respectively. Two alternative splice variants, lacking the V‑type domain or the cytoplasmic tail, are known (1, 4). RAGE is highly expressed in the embryonic central nervous system (5). In adult tissues, RAGE is expressed at low levels in multiple tissues including endothelial and smooth muscle cells, mononuclear phagocytes, pericytes, microglia, neurons, cardiac myocytes and hepatocytes (6). The expression of RAGE is upregulated upon ligand interaction. Depending on the cellular context and interacting ligand, RAGE activation can trigger differential signaling pathways that affect divergent pathways of gene expression (1, 7). RAGE activation modulates varied essential cellular responses (including inflammation, immunity, proliferation, cellular adhesion and migration) that contribute to cellular dysfunction associated with chronic diseases such as diabetes, cancer, amyloidoses and immune or inflammatory disorders (1).

Item	Value
References	Schmidt, A. et al. (2001) J. Clin. Invest. 108:949. Chavakis, T. et al. (2003) J. Exp. Med. 198:507. Neeper, M. et al. (1992) J. Biol. Chem. 267:14998. Yonekura, H. et al. (2003) Biochem. J. 370:1097. Hori, O. et al. (1995) J. Biol. Chem. 270:25752. Brett, J. et al. (1993) Am. J. Pathol. 143:1699. Valencia, J.V. et al. (2004) Diabetes 53:743.
Long Name	Receptor for Advanced Glycation End Products
Entrez Gene IDs	177 (Human); 11596 (Mouse); 81722 (Rat); 403168 (Canine)
Alternate Names	AGER, RAGE, RAGE isoform delta, RAGE isoform sRAGE-delta, Receptor for advanced glycosylation end products, SCARJ1, advanced glycosylation end product-specific receptor, receptor for advanced glycosylation end-products

Citations for Rat RAGE Antibody (2)

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Citations are publications that use Bio-Techne products. Selected citations for Rat RAGE Antibody include:

Species: Rat
Sample Types: Cell Lysates
Applications: Western Blot

Brodeur M et al. (2014), Reduction of advanced-glycation end products levels and inhibition of RAGE signaling decreases rat vascular calcification induced by diabetes. PLoS ONE, 9(1):e85922.
PMID: 24465790
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr

Pichiule P et al. (2007), Hypoxia-inducible factor-1 mediates neuronal expression of the receptor for advanced glycation end products following hypoxia/ischemia. J. Biol. Chem., 282(50):36330-40.
PMID: 17942394

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Customer Reviews for Rat RAGE Antibody (1)

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Rat RAGE Antibody

Name: Anonymous

Application: B/N

Sample Tested: Spleen tissue

Species: Rat

Verified Customer | Posted 02/24/2016

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Product Documents for Rat RAGE Antibody

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Rat RAGE Antibody

R&D Systems, Inc. a Bio-Techne Brand

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Applications for Rat RAGE Antibody

Rat RAGE Sandwich Immunoassay

Formulation, Preparation and Storage

Reconstitution Calculator

Background: RAGE/AGER

Citations for Rat RAGE Antibody (2)

Customer Reviews for Rat RAGE Antibody (1)

Rat RAGE Antibody

Product Documents for Rat RAGE Antibody

Product Datasheets

COA

Certificate of Analysis

SDS

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