Rat PBEF/Visfatin Alexa Fluor™ Plus 488-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # AF6384AFP488

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AF6384AFP488-100UG
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Key Product Details

Species Reactivity

Rat

Applications

Western Blot

Label

Alexa Fluor Plus 488 (Excitation = 493 nm, Emission = 518 nm)

Antibody Source

Polyclonal Goat IgG

View all PBEF/Visfatin Antibodies »

Product Specifications

Specificity

Detects rat PBEF/Visfatin in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross‑reactivity with recombinant mouse PBEF and recombinant human PBEF is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications

Application
Recommended Usage

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Background: PBEF/Visfatin

PBEF (pre-B cell colony-enhancing factor; also visfatin and nicotinamide phosphoribosyltransferase/Nampt) is a 52‑54 kDa member of the NAPRTase family of molecules. It is a dimer that is found both intracellularly and extracellularly, where it participates in NAD synthesis and induces proinflammatory cytokine synthesis, respectively. PBEF has also been reported to show insulin-like effects on adipocytes by binding to the insulin receptor. Cells known to express PBEF include visceral adipocytes, neutrophils, and capillary endothelium. Rat PBEF is 491 amino acids in length. It contains no signal sequence but is secreted. Potential phosphorylation sites exist at Tyr34, Tyr188 and Ser398. Full‑length (aa 1‑491) rat PBEF shares 99% and 95% aa identity with mouse and human PBEF, respectively.

Long Name

Pre-B-Cell Colony-Enhancing Factor 1

Alternate Names

NAmPRTase, NAMPT, NMPRTase, PBEF1, Visfatin

Entrez Gene IDs

10135 (Human); 59027 (Mouse); 297508 (Rat)

Gene Symbol

NAMPT

UniProt

Q80Z29

Additional PBEF/Visfatin Products

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Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices


This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

For research use only

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