Rat Neuropilin-1 Antibody

Name: Rat Neuropilin-1 Antibody
Brand: R&D Systems
SKU: MAB5661
Price: 160 EUR

R&D Systems, Inc. a Bio-Techne Brand

Catalog # MAB5661

Citations (3)

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Species Reactivity:	Rat
Immunogen:	Mouse myeloma cell line NS0-derived recombinant rat Neuropilin‑1 Met1-Asp854 (Lys811Arg, Pro812-Gly828 del), predicted Accession # Q9QWJ9
Applications:	Immunohistochemistry, Western Blot
Label:	Unconjugated
Source:	Monoclonal Mouse IgG₁ Clone # 130604
Specificity:	Detects rat Neuropilin-1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant rat Neuropilin-2 is observed.

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Applications for Rat Neuropilin-1 Antibody

	Recommended Concentration	Sample
Western Blot	1 µg/mL	Recombinant Rat Neuropilin‑1/BDCA4 Fc Chimera (Catalog # 566-N1)
Immunohistochemistry	8-25 µg/mL	Immersion fixed paraffin-embedded sections of rat embryo (E15)

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Formulation, Preparation and Storage

Purification	Protein A or G purified from hybridoma culture supernatant
Reconstitution	Reconstitute at 0.5 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: Neuropilin-1

Neuropilin-1 (Npn-1, previously neuropilin; also CD304) is a 130‑140 kDa type I transmembrane (TM) glycoprotein that regulates axon guidance and angiogenesis (1‑4). The mature 901 amino acid (aa) rat Npn-1 contains a 623 aa extracellular domain (ECD) that shares 98% aa identity with mouse and 93% aa identity with human, equine, bovine and canine Npn-1 (3, 4). The ECD contains two N-terminal CUB domains, two F5/8 type C domains with homology to coagulation factors V and VIII and a MAM (meprin) domain. In mouse and human, splice variants that lack the TM domain have been described and are either proven or presumed to be soluble antagonists (1, 5‑7). The sema domains of Class III secreted semaphorins such as Sema3A bind Npn-1 CUB domains (8). The heparin-binding forms of VEGF (VEGF₁₆₅, VEGF-B and VEGF-E), PlGF (PlGF2), and the C-terminus of Sema3 bind the F5/8 type C domains (8, 9). Npn-1 and Npn-2 share 48% aa identity within the ECD and can form homo- and hetero-oligomers via interaction of their MAM domains (1). Neuropilins show partially overlapping expression in neuronal and endothelial cells during development (1, 2). Both neuropilins act as co-receptors with plexins, mainly plexin A3 and A4, to bind class III semaphorins that mediate axon repulsion (10). However, only Npn-1 binds Sema3A, and only Npn-2 binds Sema3F (1). Both are co-receptors with VEGF R2 (also called KDR or Flk-1) for VEGF₁₆₅ binding (1). Sema3A signaling can be blocked by VEGF₁₆₅, which has higher affinity for Npn-1 (11). Npn-1 is preferentially expressed in developing or remodeling arteries (1, 2). Npn-1 is also expressed on dendritic cells and mediates DC-induced T cell proliferation (12).

Item	Value
References	Bielenberg, D.R. et al. (2006) Exp. Cell Res. 312:584. Gu, C. et al. (2003) Dev. Cell 5:45. He, Z. and M. Tessier-Lavigne (1997) Cell 90:739. Soker, S. et al. (1998) Cell 92:735. Gagnon, M.L. et al. (2000) Proc. Natl. Acad. Sci. USA 97:2573. Cackowski, F.C. et al. (2004) Genomics 84:82. Rossignol, M. et al. (2000) Genomics 70:211. Gu, C. et al. (2002) J. Biol. Chem. 277:18069. Mamluk, R. et al. (2002) J. Biol. Chem. 277:24818. Yaron, A. et al. (2005) Neuron 45:513. Narazaki, M. and G. Tosato (2006) Blood 107:3892. Tordjman, R. et al. (2002) Nat. Immunol. 3:477.
Entrez Gene IDs	8829 (Human); 18186 (Mouse); 246331 (Rat); 100286859 (Porcine)
Alternate Names	BDCA-4, BDCA4, CD304, CD304 antigen, DKFZp686A03134, DKFZp781F1414, NP1, NRP, NRP1, Neuropilin-1, Neuropilin1, VEGF165R, Vascular endothelial cell growth factor 165 receptor, neuropilin 1, transmembrane receptor

Citations for Rat Neuropilin-1 Antibody (3)

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Citations are publications that use Bio-Techne products. Selected citations for Rat Neuropilin-1 Antibody include:

Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

GJ Prud'homme et al. (2016), Neuropilin-1 is a receptor for extracellular miRNA and AGO2/miRNA complexes and mediates the internalization of miRNAs that modulate cell function Oncotarget, 7(42):68057-68071.
PMID: 27486976
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

Weiss A et al. (2014), Nephric duct insertion requires EphA4/EphA7 signaling from the pericloacal mesenchyme. Development, 141(17):3420-30.
PMID: 25139858
Species: Mouse
Sample Types: In Vivo
Applications: Neutralization

Acevedo LM et al. (2008), Semaphorin 3A suppresses VEGF-mediated angiogenesis yet acts as a vascular permeability factor. Blood, 111(5):2674-80.
PMID: 18180379

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Rat Neuropilin-1 Antibody

R&D Systems, Inc. a Bio-Techne Brand

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