Rat MCAM/CD146 APC-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB3250A

Key Product Details

Species Reactivity

Rat

Applications

Flow Cytometry

Label

Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)

Antibody Source

Monoclonal Mouse IgG2A Clone # 404722

View all MCAM/CD146 Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant rat MCAM/CD146
Arg19-Lys560
Accession # Q9EPF2

Specificity

Detects rat MCAM/CD146 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) MCAM, rhBCAM, or rhALCAM is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Rat MCAM/CD146 APC-conjugated Antibody

Detection of MCAM/CD146 antibody in PC-12 Rat Cell Line antibody by Flow Cytometry.

Detection of MCAM/CD146 in PC‑12 Rat Cell Line by Flow Cytometry.

PC-12 rat adrenal pheochromocytoma cell line was stained with Mouse Anti-Rat MCAM/CD146 APC-conjugated Monoclonal Antibody (Catalog # FAB3250A, filled histogram) or isotype control antibody (Catalog # IC003A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Rat MCAM/CD146 APC-conjugated Antibody

Application
Recommended Usage

Flow Cytometry

0.25-1 µg/106 cells
Sample: PC‑12 rat adrenal pheochromocytoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: MCAM/CD146

Melanomia Cell Adhesion Molecule (MCAM), also known as CD146 and MUC18, is a putative Ig-superfamily adhesion molecule that is expressed on endothelial cells and a variety of tumor cells. MCAM is associated with tumor progression and metastasis and may be involved in embryonic neural development. An alternately spliced isoform of rat MCAM has a 40 residue deletion in the cytoplasmic domain. Within the extracellular region, rat MCAM shares 72% and 90% amino acid sequence identity with human and mouse MCAM, respectively.

Long Name

Melanoma Cell Adhesion Molecule

Alternate Names

CD146, L-Gicerin, MUC18

Entrez Gene IDs

4162 (Human); 84004 (Mouse); 78967 (Rat)

Gene Symbol

MCAM

UniProt

Q9EPF2

Additional MCAM/CD146 Products

Product Documents for Rat MCAM/CD146 APC-conjugated Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Rat MCAM/CD146 APC-conjugated Antibody

For research use only

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