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Key Product Details

Species Reactivity

Rat

Applications

Immunohistochemistry, Western Blot

Label

Alexa Fluor 405 (Excitation = 405 nm, Emission = 421 nm)

Antibody Source

Polyclonal Sheep IgG

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant rat LYVE-1
Thr53-Thr259
Accession # NP_001099756

Specificity

Detects rat LYVE-1 in direct ELISA and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant mouse LYVE-1 is observed, and approximately 10% cross-reactivity with recombinant human LYVE-1 is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Applications for Rat LYVE-1 Alexa Fluor® 405-conjugated Antibody

Application
Recommended Usage

Immunohistochemistry

Optimal dilution of this antibody should be experimentally determined.

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: LYVE-1

LYVE-1 (Lymphatic vessel endothelial hyaluronic acid receptor 1; also CRSBP-1) is a 58-64 kDa, monomeric, glycoprotein member of the Link protein superfamily of hyaluron-binding molecules. It has limited expression, being found on the cell surface of lymphatic endothelial cells, endothelial cells of lymphoid sinuses, nodal stromal cells, and macrophages plus dendritic cells. HA (hyaluronan) is a nonsulfated, freestanding, repeating disaccharide consisting of GlcA (glucuronic acid) in beta‑linkage with GlcNAc (N-acetylglucosamine). It should not be confused with heparan, which is sulfated, protein-linked, and composed of repeating GlcA/IdoA and GlcNAc residues in both alpha- and beta-linkages. HA is ubiquitous and occupies space between collagen fibers. It undergoes both normal, and pathology-induced turnover, and presumably does so by binding to LYVE-1, CD44 and HARE on lymphatic endothelium. Ultimately, HA is transported to the liver and nodes where it undergoes degradation. This may be necessary as low MW HA is proinflammatory. LYVE-1 is also a receptor for PDGF-BB and VEGF-A, and LYVE-1 ligation apparently induces endothelial cell contraction with the opening of intercellular junctions. Based on mouse, rat LYVE-1 is synthesized as a 343 amino acid (aa) type I transmembrane protein that contains a very long signal sequence (aa 1-52). The extracellular region is likely to be 182 aa in length (aa 53-234) and contain one Link domain (aa 62‑210). LYVE-12 is likely maintained in a default "off mode" by undergoing sialylation, possibly at Thr83. Over aa 53-259, rat LYVE-1 shares 82% and 60% aa sequence identity with mouse and human LYVE-1, respectively.

Long Name

Lymphatic Vessel Endothelial Hyaluronan Receptor 1

Alternate Names

LYVE1, XLKD1

Entrez Gene IDs

10894 (Human); 114332 (Mouse); 293186 (Rat)

Gene Symbol

LYVE1

UniProt

Additional LYVE-1 Products

Product Documents for Rat LYVE-1 Alexa Fluor® 405-conjugated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Rat LYVE-1 Alexa Fluor® 405-conjugated Antibody


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

For research use only

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