Rat IL-7R alpha/CD127 PE-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # FAB8484P
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Rat
Cited:
Rat
Applications
Validated:
Flow Cytometry
Cited:
Flow Cytometry
Label
Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)
Antibody Source
Monoclonal Mouse IgG2A Clone # 717519
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant rat IL-7 R alpha/CD127
Glu21-Asp239
Accession # NP_001099888
Glu21-Asp239
Accession # NP_001099888
Specificity
Detects rat IL-7 R alpha/CD127 in direct ELISAs.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Rat IL-7R alpha/CD127 PE-conjugated Antibody
Detection of IL-7 R alpha/CD127 in Rat Splenocytes by Flow Cytometry.
Rat splenocytes were stained with Anti-Rat CD4 APC-conjugated Antibody and either (A) Mouse Anti-Rat IL-7 Ra/CD127 PE-conjugated Monoclonal Antibody (Catalog # FAB8484P) or (B) Mouse IgG2APhycoerythrin Isotype Control (Catalog # IC003P). View our protocol for Staining Membrane-associated Proteins.Applications for Rat IL-7R alpha/CD127 PE-conjugated Antibody
Application
Recommended Usage
Flow Cytometry
10 µL/106 cells
Sample: Rat splenocytes
Sample: Rat splenocytes
Spectra Viewer
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
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Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: IL-7R alpha/CD127
IL-7 R alpha (IL-7 Receptor alpha), also known as CD127, is a 60-70 kDa member of the type I cytokine receptor family of molecules. It is expressed on resting naïve and memory CD8+ T cells, DN thymocytes, pre-B cells, Th1 CD4+ T cells, dendritic cells and monocytes. IL-7 R alpha heterodimerizes with both the gammac chain to create the IL-7 receptor, and the TSLPR subunit to generate the TSLP Receptor. IL-7 R alpha participates in T cell differentiation, naïve T cell survival, and CD4+ thymocyte proliferation. Mature rat IL-7 R alpha is a 437 amino acid (aa) type I transmembrane glycoprotein. It contains a 219 aa extracellular region (aa 21-239) that possesses one type III Fibronectin domain (aa 129-222), and a 193 aa cytoplasmic tail that shows a key phosphorylation site at Tyr447. Over aa 21-239, rat IL-7 R alpha shares 79% and 67% aa identity with mouse and human IL-7 R alpha, respectively.
Long Name
Interleukin 7 Receptor alpha
Alternate Names
CD127, IL-7 R alpha, IL-7Ra, IL7R, IL7R alpha
Gene Symbol
IL7R
UniProt
Additional IL-7R alpha/CD127 Products
Product Documents for Rat IL-7R alpha/CD127 PE-conjugated Antibody
Product Specific Notices for Rat IL-7R alpha/CD127 PE-conjugated Antibody
For research use only
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