Rat GABAB R1 Alexa Fluor® 700-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # FAB7000N
Conjugate
Catalog #
Key Product Details
Species Reactivity
Rat
Applications
Immunohistochemistry
Label
Alexa Fluor 700 (Excitation = 675-700 nm, Emission = 723 nm)
Antibody Source
Monoclonal Mouse IgG2A Clone # 751216
Product Specifications
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant rat GABAB R1
Gly17-Leu586
Accession # Q9Z0U4
Gly17-Leu586
Accession # Q9Z0U4
Specificity
Detects rat GABAB R1 in direct ELISAs.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Applications
Application
Recommended Usage
Immunohistochemistry
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
Background: GABA-B R1
GABAB R1 (GABA-B receptor subunit 1; also GABA-BR1, GABBR1 and GB1) is a multispan glycoprotein in the GABA-B receptor subfamily, GPCR-3 family of proteins. It forms an obligatory heterodimer with GABA-BR2, creating a G-protein metabotropic GABA receptor that inhibits adenylyl cyclase activity and activates K+ channels. Presynaptically, this blocks neurotransmitter release; postsynaptically, it lowers neuron excitability. Rat GABAB R1 is 991 amino acids (aa) in length. It contains a 16 aa signal sequence, an extended N‑terminal extracellular region (aa 17-590) that contains two SUSHI domains (aa 29‑158), and a long C-terminal cytoplasmic domain (aa 885-991). There are several splice variants with predicted molecular weights ranging from 90 to 111 kDa and multiple glycosylation sites. The 991 aa isoform described above is called GABAB R1e (R1e). There is also a 960 aa, 130 kDa isoform that shows a deletion of aa 771‑801. This variant (R1a) is associated with postsynaptic membranes. A third isoform (R1b) is 844 aa in length and 100 kDa in size and possesses both a deletion of aa 771-801 and a 47 aa substitution for aa 1-163. This variant is presynaptic in location. Two other isoforms are variants of GABAB R1b. Each show the same N‑terminal substitution, with a fourth isoform (R1c) retaining aa 771-801 and a fifth isoform (R1d) deleting aa 771-801, coupled to a 25 aa substitution for aa 935‑991. Over aa 17-586, rat GABAB R1e/a shares 99% aa identity with both mouse and human GABAB R1.
Long Name
gamma-Aminobutyric Acid Type B Receptor Subunit 1
Alternate Names
GABABR1, GABBR1, Gb1, GPRC3A
Gene Symbol
GABBR1
UniProt
Additional GABA-B R1 Products
Product Specific Notices
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
For research use only
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