Rat FGF‑BP Antibody

Name: Rat FGF‑BP Antibody
Brand: R&D Systems
SKU: AF1413

Catalog # AF1413 | R&D Systems, Inc. a Bio-Techne Brand

Citations (1)

Product Datasheet / COA / SDS

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Product Summary
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Rat

Applications

Blockade of Receptor-ligand Interaction, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Product Summary for Rat FGF‑BP Antibody

Immunogen

Mouse myeloma cell line NS0-derived recombinant rat FGF-BP
Glu21-Cys238
Accession # Q9QY10

Specificity

Detects rat FGF-BP in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 10% cross-reactivity with recombinant human FGF-BP is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Rat FGF‑BP Antibody

FGF-BP in Rat Thymus.

FGF-BP was detected in perfusion fixed frozen sections of rat thymus using Rat FGF-BP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1413) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of lymphocytes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Applications for Rat FGF‑BP Antibody

Application

Recommended Usage

Blockade of Receptor-ligand Interaction

In a functional ELISA, 0.5-2 µg/mL of this antibody will block 50% of the binding of 50 ng/mL of Recombinant Rat FGF-BP (Catalog # 1413-FB) to immobilized Recombinant Human FGF-basic (Catalog # 233-FB) coated at 5 µg/mL (100 µL/well). At 10 μg/mL, this antibody will block >90% of the binding.

Immunohistochemistry

5-15 µg/mL
Sample: Perfusion fixed frozen sections of rat thymus

Western Blot

0.1 µg/mL
Sample: Recombinant Rat FGF-BP (Catalog # 1413-FB)

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Published Applications

Read 1 publication using AF1413 in the following applications:

IHC-Fr (1 Publication)

Formulation, Preparation and Storage

Purification

Antigen Affinity-purified

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FGF-BP

Fibroblast growth factor binding protein (FGF-BP), also known as HBp17, is a secreted glycoprotein that increases the bioavailability of FGFs (1). Mature FGF-BP is a 34 kDa O-glycosylated protein with five conserved intrachain disulfide bonds (2-4). FGF-BP contains a heparin-binding domain (aa 113-146) and a distinct FGF-binding region (aa 197-238) (5). Mature rat FGF-BP shares 54% and 81% aa sequence identity with mouse and rat FGF-BP, respectively. FGF-BP is expressed throughout development and in adult squamous epithelium (6, 7). It is upregulated in injured skin, renal tubular epithelium, and spinal nerves as well as in carcinomas of the skin, colon, and pancreas (3, 8-11). FGF-BP binds FGF -1, -2, -7, -10, and -22 which are secreted and sequestered in the extracellular matrix (ECM) (8, 12). The interactions of FGF-BP with heparin sulfate proteoglycans (HSPG) and FGF, modulates their activities (7, 9 13, 14). FGF-BP enhances the mitogenic effects of FGFs, thereby contributing to epithelial, endothelial, and neuronal tissue repair, angiogenesis, and tumor growth (8-10, 12, 15, 16).

References

Abuharbeid, S. et al. (2006) Int. J. Biochem. Cell Biol. 38:1463.
Aigner, A. et al. (2000) Growth Factors 18:51.
Tassi, E. et al. (2006) Cancer Res. 66:1191.
Lametsch, R. et al. (2000) J. Biol. Chem. 275:19469.
Xie, B. et al. (2006) J. Biol. Chem. 281:1137.
Aigner, A. et al. (2002) Histochem. Cell Biol. 117:1.
Wu, D. et al. (1991) J. Biol. Chem. 266:16778.
Beer, H-D. et al. (2005) Oncogene 24:5269.
Ray, P.E. et al. (2006) Am. J. Physiol. Regul. Integr. Comp. Physiol. 290:R105.
Tassi, E. et al. (2007) Am. J. Physiol. Regul. Integr. Comp. Physiol. 293:R775.
Kurtz, A. et al. (2004) Neoplasia 6:595.
Tassi, E. et al. (2001) J. Biol. Chem. 276:40247.
Mongiat, M. et al. (2001) J. Biol. Chem. 276:10263.
Kurtz, A. et al. (1997) Oncogene 14:2671.
Aigner, A. et al. (2001) Int. J. Cancer 92:510.
Czubayko, F. et al. (1997) Nat. Med. 3:1137.

Long Name

Fibroblast Growth Factor Binding Protein

Alternate Names

FGF-BP1, FGFBP, FGFBP-1, FGFBP1, HBp17

Entrez Gene IDs

9982 (Human); 14181 (Mouse); 64535 (Rat)

Gene Symbol

FGFBP1

UniProt

Q9QY10

Product Documents for Rat FGF‑BP Antibody

Product Datasheets

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COA

SDS

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Product Specific Notices for Rat FGF‑BP Antibody

For research use only

Citations for Rat FGF‑BP Antibody (1)

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Citations are publications that use Bio-Techne products. Selected citations for Rat FGF‑BP Antibody include:

Species: Rat
Sample Types: Whole Tissue
Applications: IHC-Fr

K Nagashima et al. (2017-10-18), Priming with FGF2 stimulates human dental pulp cells to promote axonal regeneration and locomotor function recovery after spinal cord injury Sci Rep, 2017-17(1):13500.
PMID: 29044129

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