Rat FGF‑BP Antibody

Catalog # MAB1413 | R&D Systems, Inc. a Bio-Techne Brand
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MAB1413
MAB1413-SP
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Key Product Details

Species Reactivity

Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 227239

Product Summary for Rat FGF‑BP Antibody

Immunogen

Mouse myeloma cell line NS0-derived recombinant rat FGF-BP
Glu21-Cys238
Accession # Q9QY10

Specificity

Detects rat FGF-BP in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human FGF-BP is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Applications for Rat FGF‑BP Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Rat FGF-BP (Catalog # 1413-FB)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FGF-BP

Fibroblast growth factor binding protein (FGF-BP), also known as HBp17, is a secreted glycoprotein that increases the bioavailability of FGFs (1). Mature FGF-BP is a 34 kDa O-glycosylated protein with five conserved intrachain disulfide bonds (2‑4). FGF-BP contains a heparin-binding domain (aa 113‑146) and a distinct FGF-binding region (aa 197‑238) (5). Mature rat FGF-BP shares 54% and 81% aa sequence identity with mouse and rat FGF-BP, respectively. FGF-BP is expressed throughout development and in adult squamous epithelium (6, 7). It is upregulated in injured skin, renal tubular epithelium, and spinal nerves as well as in carcinomas of the skin, colon, and pancreas (3, 8‑11). FGF-BP binds FGF -1, -2, -7, -10, and -22 which are secreted and sequestered in the extracellular matrix (ECM) (8, 12). The interactions of FGF-BP with heparin sulfate proteoglycans (HSPG) and FGF, modulates their activities (7, 9 13, 14). FGF-BP enhances the mitogenic effects of FGFs, thereby contributing to epithelial, endothelial, and neuronal tissue repair, angiogenesis, and tumor growth (8‑10, 12, 15, 16).

 

References

  1. Abuharbeid, S. et al. (2006) Int. J. Biochem. Cell Biol. 38:1463.
  2. Aigner, A. et al. (2000) Growth Factors 18:51.
  3. Tassi, E. et al. (2006) Cancer Res. 66:1191.
  4. Lametsch, R. et al. (2000) J. Biol. Chem. 275:19469.
  5. Xie, B. et al. (2006) J. Biol. Chem. 281:1137.
  6. Aigner, A. et al. (2002) Histochem. Cell Biol. 117:1.
  7. Wu, D. et al. (1991) J. Biol. Chem. 266:16778.
  8. Beer, H.-D. et al. (2005) Oncogene 24:5269.
  9. Ray, P.E. et al. (2006) Am. J. Physiol. Regul. Integr. Comp. Physiol. 290:R105.
  10. Tassi, E. et al. (2007) Am. J. Physiol. Regul. Integr. Comp. Physiol. 293:R775.
  11. Kurtz, A. et al. (2004) Neoplasia 6:595.
  12. Tassi, E. et al. (2001) J. Biol. Chem. 276:40247.
  13. Mongiat, M. et al. (2001) J. Biol. Chem. 276:10263.
  14. Kurtz, A. et al. (1997) Oncogene 14:2671.
  15. Aigner, A. et al. (2001) Int. J. Cancer 92:510.
  16. Czubayko, F. et al. (1997) Nat. Med. 3:1137.

Long Name

Fibroblast Growth Factor Binding Protein

Alternate Names

FGF-BP1, FGFBP, FGFBP-1, FGFBP1, HBp17

Entrez Gene IDs

9982 (Human); 14181 (Mouse); 64535 (Rat)

Gene Symbol

FGFBP1

UniProt

Q9QY10

Product Documents for Rat FGF‑BP Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Rat FGF‑BP Antibody

For research use only

Reconstitution Calculator

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