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Rat beta-NGF Antibody

Catalog # MAB556 | R&D Systems, Inc. a Bio-Techne Brand
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MAB556
MAB556-SP

Key Product Details

Species Reactivity

Rat

Applications

Neutralization

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 884530

Product Summary for Rat beta-NGF Antibody

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant rat beta‑NGF
Met1-Gly241
Accession # P25427

Specificity

Detects rat beta‑NGF in ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Rat beta-NGF Antibody

Proliferation Induced by  beta-NGF and Neutralization by Rat  beta‑NGF Antibody.

Proliferation Induced by beta-NGF and Neutralization by Rat beta‑NGF Antibody.

Recombinant Rat beta-NGF(Catalog # 556-NG) induces proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line), as measured by Rezazurin (Catalog # AR002). Proliferation elicited by Recombinant Rat beta-NGF (10 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Rat beta-NGF Monoclonal Antibody (Catalog # MAB556). The ND50 is typically 0.05-0.25 µg/ml.

Applications for Rat beta-NGF Antibody

Application
Recommended Usage

Neutralization

Measured by its ability to neutralize beta‑NGF-induced proliferation in the TF‑1 human erythroleukemic cell line. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323.The Neutralization Dose (ND50) is typically 0.05-0.25 μg/ml in the presence of 10 ng/mL Recombinant Rat beta‑NGF.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Published Applications

Read 1 publication using MAB556 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: beta-NGF

NGF was initially isolated in the mouse submandibular gland over three decades ago as a 7S complex composed of three non-covalently linked subunits, alpha, beta, and gamma. It is now known that both the alpha and gamma subunits of NGF are members of the kallikrein family of serine proteases while the beta subunit, called beta-NGF or 2.5S NGF, exhibits all the biological activities ascribed to NGF. Recombinant rat beta-NGF is a homodimer of two 120-amino acid polypeptides. The human protein shares approximately 90% homology at the amino acid level with both the mouse and rat beta-NGF and exhibits cross-species activity.

NGF is a well-characterized neurotrophic protein that plays a critical role in the development of sympathetic and some sensory neurons in the peripheral nervous system. In addition, NGF can also act in the central nervous system as a trophic factor for basal forebrain cholinergic neurons. NGF has also been shown to have biological effects on non-neuronal tissues. NGF is mitogenic for a factor‑dependent human erythroleukemic cell line, TF-1. NGF has been found to increase the number of mast cells in neonatal rats and to induce histamine release from peritoneal mast cells. NGF will enhance histamine release and strongly modulate the formation of lipid mediators by basophils in response to various stimuli. NGF will also induce the growth and differentiation of human B lymphocytes as well as suppress apoptosis of murine peritoneal neutrophils. These results, taken together, suggest that NGF is a pleiotropic cytokine which, in addition to its neurotropic activities, may have an important role in the regulation of the immune system.

Long Name

beta Nerve Growth Factor

Alternate Names

betaNGF, NGFB

Entrez Gene IDs

4803 (Human); 18049 (Mouse); 310738 (Rat); 403402 (Canine); 103350089 (Rabbit)

Gene Symbol

NGF

UniProt

Product Documents for Rat beta-NGF Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Rat beta-NGF Antibody

For research use only

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Reconstitution Calculator

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