Rat alpha2u-Globulin Antibody
R&D Systems, part of Bio-Techne | Catalog # AF586
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Conjugate
Catalog #
Key Product Details
Species Reactivity
Rat
Applications
Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Rat urine-derived alpha2u-Globulin
Specificity
Detects rat alpha
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Rat alpha2u-Globulin Antibody
alpha2u-Globulin in Rat Liver.
a2u-Globulin was detected in perfusion fixed frozen sections of rat liver using 5 µg/mL Goat Anti-Rat a2u-Globulin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF586) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.Applications for Rat alpha2u-Globulin Antibody
Application
Recommended Usage
Immunohistochemistry
5-15 µg/mL
Sample: Perfusion fixed frozen sections of rat liver
Sample: Perfusion fixed frozen sections of rat liver
Western Blot
0.1 µg/mL
Sample: Rat alpha2u-Globulin
Sample: Rat alpha2u-Globulin
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: alpha 2u-Globulin
Rat alpha 2u-Globulin (AUG), also known as major urinary protein, belongs to the lipocalin protein family. Under complex hormonal control, alpha 2u-globulin is a low molecular weight protein expressed in a tissue-, age-, and sex-specific fashion. Circulating alpha 2u-globulin is expressed by the liver. It is removed from the plasma by the kidney and excreted.
Rodent studies have employed alpha 2u-globulin nephropathy as an experimental model for the development of kidney tumors. Induction of alpha 2u-globulin nephropathy alters the number, size and shape of hyaline droplets in the proximal tubules. Intra-cellular accumulation of alpha 2u-globulin induces cell death and necrosis of tubular epithelial cells.
Alternate Names
AUG, PGCL1
Additional alpha 2u-Globulin Products
Product Documents for Rat alpha2u-Globulin Antibody
Product Specific Notices for Rat alpha2u-Globulin Antibody
For research use only
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