Rad51 Antibody (23GB2275)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-51031
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Knockdown Validated
Label
Unconjugated
Antibody Source
Monoclonal Rabbit IgG Clone # 23GB2275
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthesized peptide derived from human Rad51 (Uniprot: Q06609)
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Rad51 Antibody (23GB2275)
Immunocytochemistry/ Immunofluorescence: Rad51 Antibody (23GB2275)[NBP3-51031] -
Immunocytochemical staining of HAP-1 cells with Rad51 antibody (1:1,000). Nuclei were stained blue with DAPI; Rad51 was stained magenta with Alexa Fluor(R) 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 um.
Flow Cytometry: Rad51 Antibody (23GB2275)[NBP3-51031] -
Flow cytometric analysis of Rad51expression in HAP-1 cells using Rad51 antibody (1:2,000). Green, isotype control; red, RAD51.
Western Blot: Rad51 Antibody (23GB2275)[NBP3-51031] -
Western blotting analysis using Rad51 antibody (1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using FeQ(TM) ECL Substrate Kit.Applications for Rad51 Antibody (23GB2275)
Application
Recommended Usage
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Knockdown Validated
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.4), 50% glycerol
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Rad51
Rad51 is involved in both the recombination and repair of DNA damage. Its function may be modulated by interaction with other repair proteins including BRCA1 and BRCA2. Alterations in the Rad51 gene have been associated with increased cancer risk. Rad51 is conserved across species and is homologous to prokaryotic RecA.
Alternate Names
BRCC5, hRAD51, HsRad51, HsT16930, RAD51 (S. cerevisiae) homolog (E coli RecA homolog), RAD51 homolog (RecA homolog, E. coli) (S. cerevisiae), RAD51 homolog A, RAD51ABRCA1/BRCA2-containing complex, subunit 5, RAD51L3, RecA, E. coli, homolog of, RECADNA repair protein RAD51 homolog 1, RecA-like protein, recombination protein A
Gene Symbol
RAD51
Additional Rad51 Products
Product Documents for Rad51 Antibody (23GB2275)
Product Specific Notices for Rad51 Antibody (23GB2275)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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