Rad23 Antibody (3B7T9)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16573
Recombinant Monoclonal Antibody
![Immunocytochemistry/ Immunofluorescence: Rad23 Antibody (3B7T9) [NBP3-16573]](https://resources.bio-techne.com/images/products/Rad23-Antibody-3B7T9-Immunocytochemistry-Immunofluorescence-NBP3-16573-img0001.jpg "Immunocytochemistry/ Immunofluorescence: Rad23 Antibody (3B7T9) [NBP3-16573]")
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 3B7T9 expressed in HEK293
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 200-300 of human Rad23 (P54725). TGIPGSPEPEHGSVQESQVSEQPATEAAGENPLEFLRDQPQFQNMRQVIQQNPALLPALLQQLGQENPQLLQQISRHQEQFIQMLNEPPGELADISDVEGE
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Description
Novus Biologicals Rabbit Rad23 Antibody (3B7T9) (NBP3-16573) is a recombinant monoclonal antibody validated for use in WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for Rad23 Antibody (3B7T9)
![Western Blot: Rad23 Antibody (3B7T9) [NBP3-16573]](https://resources.bio-techne.com/images/products/Rad23-Antibody-3B7T9-Western-Blot-NBP3-16573-img0003.jpg "Western Blot: Rad23 Antibody (3B7T9) [NBP3-16573]")
Western Blot: Rad23 Antibody (3B7T9) [NBP3-16573]
Western Blot: Rad23 Antibody (3B7T9) [NBP3-16573] - Western blot analysis of extracts of various cell lines, using Rad23 Rabbit mAb (NBP3-16573) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.![Immunocytochemistry/ Immunofluorescence: Rad23 Antibody (3B7T9) [NBP3-16573]](https://resources.bio-techne.com/images/products/Rad23-Antibody-3B7T9-Immunocytochemistry-Immunofluorescence-NBP3-16573-img0002.jpg "Immunocytochemistry/ Immunofluorescence: Rad23 Antibody (3B7T9) [NBP3-16573]")
Immunocytochemistry/ Immunofluorescence: Rad23 Antibody (3B7T9) [NBP3-16573]
Immunocytochemistry/Immunofluorescence: Rad23 Antibody (3B7T9) [NBP3-16573] - Immunofluorescence analysis of U-2 OS cells using Rad23 Rabbit mAb (NBP3-16573) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.![Western Blot: Rad23 Antibody (3B7T9) [NBP3-16573]](https://resources.bio-techne.com/images/products/Rad23-Antibody-3B7T9-Western-Blot-NBP3-16573-img0004.jpg "Western Blot: Rad23 Antibody (3B7T9) [NBP3-16573]")
Western Blot: Rad23 Antibody (3B7T9) [NBP3-16573]
Western Blot: Rad23 Antibody (3B7T9) [NBP3-16573] - Western blot analysis of extracts of various cell lines, using Rad23 Rabbit mAb (NBP3-16573) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.Applications for Rad23 Antibody (3B7T9)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50 - 1:200
Western Blot
1:500 - 1:2000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol, 0.05% BSA
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Rad23
Rad23 is an evolutionarily conserved protein that is important for nucleotide excision repair. All eukaryotic Rad23 proteins contain an amino-terminal UbL domain that bears a striking resemblance to ubiquitin. Rad23 has a proteolytic function in DNA repair via its interaction with the 26S proteosome through the UbL domain. The elimination of this Rad23 domain causes sensitivity to ultraviolet radiation.
Alternate Names
hHR23
Gene Symbol
RAD23A
Additional Rad23 Products
Product Documents for Rad23 Antibody (3B7T9)
Product Specific Notices for Rad23 Antibody (3B7T9)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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