Rab7a Antibody (5A10A4)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15444
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 5A10A4 expressed in HEK293
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 108-207 of human Rab7a (NP_004628.4). IQASPRDPENFPFVVLGNKIDLENRQVATKRAQAWCYSKNNIPYFETSAKEAINVEQAFQTIARNALKQETEVELYNEFPEPIKLDKNDRAKASAESCSC
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
23 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Rab7a Antibody (5A10A4)
![Western Blot: Rab7a Antibody (5A10A4) [NBP3-15444]](https://resources.bio-techne.com/images/products/Rab7a-Antibody-4X3P6-Western-Blot-NBP3-15444-img0002.jpg "Western Blot: Rab7a Antibody (5A10A4) [NBP3-15444]")
Western Blot: Rab7a Antibody (5A10A4) [NBP3-15444]
Western Blot: Rab7a Antibody (4X3P6) [NBP3-15444] - Western blot analysis of extracts of various cell lines, using Rab7a Rabbit mAb (NBP3-15444) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Western Blot: Rab7a Antibody (5A10A4) [Rab7a] -
Western Blot: Rab7a Antibody (5A10A4) [Rab7a] - Western blot analysis of various lysates using Rab7a Rabbit mAb at 1:55000 dilution incubated overnight at 4C.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25 ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 20s.
Immunocytochemistry/ Immunofluorescence: Rab7a Antibody (5A10A4) [Rab7a] -
Immunocytochemistry/ Immunofluorescence: Rab7a Antibody (5A10A4) [Rab7a] - Confocal imaging of NIH/3T3 cells using Rab7a Rabbit mAb followed by a further incubation with ABflo 488-conjugated Goat Anti-Rabbit IgG (H+L) . The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with Cy3 Goat Anti-Mouse IgG (H+L) Ab (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
Applications for Rab7a Antibody (5A10A4)
Application
Recommended Usage
ELISA
Recommended starting concentration is 1 μg/mL.
Immunocytochemistry/ Immunofluorescence
1:1000 - 1:8000
Immunohistochemistry-Paraffin
1:1000 - 1:4000
Immunoprecipitation
0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Western Blot
1:55000 - 1:220000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol, 0.05% BSA
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Rab7a
Long Name
RAs Genes from Brain Protein 7a
Alternate Names
PRO2706, RAB7
Gene Symbol
RAB7A
Additional Rab7a Products
Product Documents for Rab7a Antibody (5A10A4)
Product Specific Notices for Rab7a Antibody (5A10A4)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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