Rab4 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-18233
![Western Blot: Rab4 Antibody [NBP3-18233]](https://resources.bio-techne.com/images/products/Rab4-Antibody-Western-Blot-NBP3-18233-img0001.jpg "Western Blot: Rab4 Antibody [NBP3-18233]")
![Western Blot: Rab4 Antibody [NBP3-18233]](https://resources.bio-techne.com/images/products/Rab4-Antibody-Western-Blot-NBP3-18233-img0004.jpg "Western Blot: Rab4 Antibody [NBP3-18233]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
C-terminal peptide from human Rab4
Localization
Membrane|Cytoplasm
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Rab4 Antibody - BSA Free
Western Blot: Rab4 Antibody [NBP3-18233]
Western Blot: Rab4 Antibody [NBP3-18233] - Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of Rab4 protein using Rabbit Anti-Rab4 Polyclonal Antibody (NBP3-18233). Load: 15 ugprotein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rabbit Anti-Rab4 Polyclonal Antibody (NBP3-18233) at 1:1000 for 2 hours at RT. Secondary Antibody: Donkey Anti-Rabbit IgG: HRP for 1 hour at RT.![Immunocytochemistry/ Immunofluorescence: Rab4 Antibody [NBP3-18233]](https://resources.bio-techne.com/images/products/Rab4-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-18233-img0003.jpg "Immunocytochemistry/ Immunofluorescence: Rab4 Antibody [NBP3-18233]")
Immunocytochemistry/ Immunofluorescence: Rab4 Antibody [NBP3-18233]
Immunocytochemistry/Immunofluorescence: Rab4 Antibody [NBP3-18233] - Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Rab4 Polyclonal Antibody (NBP3-18233). Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol at -20C for 10 minutes. Primary Antibody: Rabbit Anti-Rab4 Polyclonal Antibody (NBP3-18233) at 1:100 for 12 hours at 4C. Secondary Antibody: FITC Goat Anti-Rabbit at 1:50 for 1-2 hours at RT in dark. Localization: String nuclear and cytoplasmic staining.![Immunohistochemistry: Rab4 Antibody [NBP3-18233]](https://resources.bio-techne.com/images/products/Rab4-Antibody-Immunohistochemistry-NBP3-18233-img0002.jpg "Immunohistochemistry: Rab4 Antibody [NBP3-18233]")
Immunohistochemistry: Rab4 Antibody [NBP3-18233]
Immunohistochemistry: Rab4 Antibody [NBP3-18233] - Immunohistochemistry analysis using Rabbit Anti-Rab4 Polyclonal Antibody (NBP3-18233). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative Solution. Primary Antibody: Rabbit Anti-Rab4 Polyclonal Antibody (NBP3-18233) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:50 for 1 hour at RT. Localization: Epidermis (cell-cell border and cytoplasmic), hair follicles and muscle.Applications for Rab4 Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:150
Immunohistochemistry
1:100
Western Blot
1:1000
Application Notes
Optimal dilutions for assays should be determined by the user.
Formulation, Preparation, and Storage
Purification
Peptide affinity purified
Formulation
PBS pH 7.4, 50% glycerol
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Rab4
Alternate Names
Oncogene RAB4, RAB4, member RAS oncogene family, RAB4A, member RAS oncogene family, RAB4HRES-1/RAB4, ras-related protein Rab-4A
Gene Symbol
RAB4A
Additional Rab4 Products
Product Documents for Rab4 Antibody - BSA Free
Product Specific Notices for Rab4 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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