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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 5E9

Format

BSA Free

Concentration

1 mg/ml

Product Specifications

Immunogen

Full length recombinant Rab3D protein (Accession #NP_004274)

Localization

Membrane

Specificity

Detects ~24 kDa. Does not show cross reactivity to RAB3A, RAB3B, or RAB3C.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Rab3D Antibody (5E9) - BSA Free

Western Blot: Rab3D Antibody (5E9) [NBP3-14764]

Western Blot: Rab3D Antibody (5E9) [NBP3-14764]

Western Blot: Rab3D Antibody (5E9) [NBP3-14764] - Western Blot analysis of Human HEK293 cells overexpressing RAB3A, RAB3B, RAB3C, and Rab3D showing detection of Rab3D protein using Mouse Anti-Rab3D Monoclonal Antibody, Clone 5E9 (NBP3-14764). Lane 1: MW ladder. Lanes 2-4: HEK293 cells overexpressing RAB3A. Lanes 5-7: HEK293 cells overexpressing RAB3B. Lanes 8-10: HEK293 cells overexpressing RAB3C. Lanes 11-13: HEK293 cells overexpressing Rab3D.. Primary Antibody: Mouse Anti-Rab3D Monoclonal Antibody (NBP3-14764). Courtesy of: Dario Alessi Lab, University of Dundee.
Western Blot: Rab3D Antibody (5E9) [NBP3-14764]

Western Blot: Rab3D Antibody (5E9) [NBP3-14764]

Western Blot: Rab3D Antibody (5E9) [NBP3-14764] - Western Blot analysis of Human, Mouse, Rat A549, T-47D cells, mouse and rat brain, kidney showing detection of Rab3D protein using Mouse Anti-Rab3D Monoclonal Antibody, Clone 5E9 (NBP3-14764). Lane 1: MW ladder. Lane 2: WT A549 cells. Lane 3: T-47D cells. Lane 4: mouse brain. Lane 5: rat brain. Lane 6: mouse kidney. Lane 7: rat kidney.. Load: 15 ug. Block: 5% Skim Milk Powder in TBST. Primary Antibody: Mouse Anti-Rab3D Monoclonal Antibody (NBP3-14764) at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-mouse IgG:HRP at 1:4000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT.

Applications for Rab3D Antibody (5E9) - BSA Free

Application
Recommended Usage

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS pH 7.4, 50% glycerol

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C.

Background: Rab3D

Protein transport. Probably involved in regulated exocytosis

Alternate Names

D2-2, GOVras-related protein Rab-3D, RAB16glioblastoma overexpressed, RAB3D, member RAS oncogene family, RAD3DRab3D upregulated with myeloid differentiation

Gene Symbol

RAB3D

Additional Rab3D Products

Product Documents for Rab3D Antibody (5E9) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Rab3D Antibody (5E9) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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