Rab11A Antibody (24GB820)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-50564

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NBP3-50564-100ul
NBP3-50564-20ul
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # 24GB820

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Rab11A Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from human Rab11A (Uniprot: P62491)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Rab11A Antibody (24GB820)

Rab11A Antibody (24GB820)

Immunocytochemistry/ Immunofluorescence: Rab11A Antibody (24GB820)[NBP3-50564] -

Immunocytochemical staining of HAP-1 cells with Rab11A antibody (1:1,000). Nuclei were stained blue with DAPI; Rab11A was stained magenta with Alexa Fluor(R) 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 um.
Rab11A Antibody (24GB820)

Flow Cytometry: Rab11A Antibody (24GB820)[NBP3-50564] -

Flow cytometric analysis of Rab11Aexpression in HAP-1 cells using Rab11A antibody (1:2,000). Green, isotype control; red, RAB11A.
Rab11A Antibody (24GB820)

Western Blot: Rab11A Antibody (24GB820)[NBP3-50564] -

Western blotting analysis using Rab11A antibody (1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using FeQ(TM) ECL Substrate Kit.

Applications for Rab11A Antibody (24GB820)

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.4), 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Rab11A

Rab proteins are low-molecular-weight GTP-binding proteins that form the largest branch of the Ras superfamily of GTPases. Located on the cytoplasmic face of organelles and vesicles, rab proteins are involved in intracellular membrane fusion reactions. Three membrane proteins, synaptosomal associated protein of 25 kDa (SNAP-25), synaptobrevin, and syntaxin, form the core of a ubiquitous membrane fusion machine that interacts with the soluble proteins N-ethylmaleimide-sensitive factor (NSF) and a-SNAP. Rab proteins, in coordination with the core fusion machinery and Munc-18, help to mediate vesicle docking and fusion. There are over 40 Rab proteins in mammals. Rab11a and Rab11b are known markers for protein trafficking, sorting, and recycling in the endosomal pathway. The Rab11 proteins are enriched in recycling endosomes and the trans-Golgi network, where they regulate membrane recycling back to the plasma membrane. Rab11a is ubiquitously expressed, while Rab11b is expressed mainly in the heart and brain.

Long Name

RAs Genes from Brain Protein 11A

Alternate Names

Rab-11, YL8

Gene Symbol

RAB11A

Additional Rab11A Products

Product Documents for Rab11A Antibody (24GB820)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Rab11A Antibody (24GB820)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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