Pyruvate Carboxylase Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-49536

Novus Biologicals, part of Bio-Techne

Key Product Details

Validated by

Knockout/Knockdown, Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse, Rat

Predicted:

Bovine (96%), Porcine (96%). Backed by our 100% Guarantee.

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockdown Validated, Western Blot

Cited:

IF/IHC, Immunocytochemistry, Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.0 mg/ml

View all Pyruvate Carboxylase/PC Antibodies »

Product Specifications

Immunogen

Partial recombinant protein made to an internal region of the human Pyruvate Carboxylase protein (within residues 930-1050). [Swiss-Prot P11498]

Reactivity Notes

Immunogen has 96% identity to bovine and porcine. Rat reactivity reported in scientific literature (PMID: 24333689)

Localization

Mitochondrion matrix.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

126 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Pyruvate Carboxylase Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: Pyruvate Carboxylase Antibody - BSA Free [NBP1-49536]

Immunocytochemistry/ Immunofluorescence: Pyruvate Carboxylase Antibody - BSA Free [NBP1-49536]

Immunocytochemistry/Immunofluorescence: Pyruvate Carboxylase Antibody [NBP1-49536] - HeLa cells were fixed in 4% paraformaldehyde for 10 min and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti- (NBP1-49536) at 10 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.
Western Blot: Pyruvate Carboxylase AntibodyBSA Free [NBP1-49536]

Western Blot: Pyruvate Carboxylase AntibodyBSA Free [NBP1-49536]

Western Blot: Pyruvate Carboxylase Antibody [NBP1-49536] - Analysis of Pyruvate Carboxylase in A. Human liver extracts and B. Mouse liver extracts
Immunohistochemistry-Paraffin: Pyruvate Carboxylase Antibody - BSA Free [NBP1-49536]

Immunohistochemistry-Paraffin: Pyruvate Carboxylase Antibody - BSA Free [NBP1-49536]

Immunohistochemistry-Paraffin: Pyruvate Carboxylase Antibody [NBP1-49536] - IHC analysis of a formalin fixed and paraffin embedded tissue section of mouse kidney using rabbit anti-Pyruvate Carboxylase antibody at 1:200 dilution. The signal was developed using HRP conjugated anti-rabbit secondary antibody and DAB reagent. The nuclei were counterstained using hematoxylin. This Pyruvate Carboxylase antibody generated a diffused cytoplasmic staining in all the tubules and a subset of cells in glomerular cells.
View 4 more images

Applications for Pyruvate Carboxylase Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50-1:100

Immunohistochemistry

1:200

Immunohistochemistry-Paraffin

1:200

Western Blot

1:1000. Use reported in scientific literature (PMID 29937374)
Application Notes
In Western blot, a band is seen at ~129 kDa. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Pyruvate Carboxylase/PC

Pyruvate Carboxylase (PC) catalyzes an important 2-step reaction - (i) ATP-dependent carboxylation of covalently attached biotin (ii) transfer of carboxyl group to pyruvate, and this interconversion is essential for replenishment of TCA/citric acid cycle intermediates (anaplerosis). By extension, PC participates in many pathways that depend on oxaloacetate (OAA) such as gluconeogenesis, glycogen synthesis, lipogenesis, glycerogenesis, glucose-dependent insulin secretion, and synthesis of amino acids as well as neurotransmitters. With highest expression levels in liver, adipose tissue, kidney, lactating mammary gland, pancreatic islets etc, PC localize to cellular mitochondrial matrix and its activity is highly regulated by its allosteric activator acetyl-CoA. Enhanced acetyl-CoA levels indicate a need for increased OAA synthesis to replenish TCA cycle intermediates or a diversion of pyruvate for glucose synthesis in gluconeogenic tissues. PC's deficiency causes a fatal multiorgan metabolic imbalance that predominantly manifests with lactic acidemia and neurological dysfunction at an early age. Three clinical forms of PC deficiency include an infantile or mild form (Type A), a severe neonatal form (Type B), and a benign form (Type C), all of which exhibit clinical or biochemical parallals of impaired anaplerosis.

Long Name

Pyruvate Carboxylase

Alternate Names

PC, PCB

Entrez Gene IDs

5091 (Human)

Gene Symbol

PC

UniProt

P11498 (Human), Q05920 (Mouse)

Additional Pyruvate Carboxylase/PC Products

Product Documents for Pyruvate Carboxylase Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Pyruvate Carboxylase Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.