Pygopus-1 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-42665
![Immunohistochemistry: Pygopus-1 Antibody [NBP1-42665]](https://resources.bio-techne.com/images/products/Pygopus-1-Antibody-Immunohistochemistry-NBP1-42665-img0005.jpg "Immunohistochemistry: Pygopus-1 Antibody [NBP1-42665]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Predicted:
Mouse (100%). Backed by our 100% Guarantee.
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot (Negative)
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
The immunogen for this product maps to a region between residue 300 and 350 of human pygopus homolog 1 using the numbering given in entry NP_056432.1 (GeneID 26108 ).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
Novus Biologicals Rabbit Pygopus-1 Antibody - BSA Free (NBP1-42665) is a polyclonal antibody validated for use in IHC and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for Pygopus-1 Antibody - BSA Free
Immunohistochemistry: Pygopus-1 Antibody [NBP1-42665]
Immunohistochemistry: Pygopus-1 Antibody [NBP1-42665] - Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti- Pygo1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB![Immunoprecipitation: Pygopus-1 Antibody [NBP1-42665]](https://resources.bio-techne.com/images/products/Pygopus-1-Antibody-Immunoprecipitation-NBP1-42665-img0004.jpg "Immunoprecipitation: Pygopus-1 Antibody [NBP1-42665]")
Immunoprecipitation: Pygopus-1 Antibody [NBP1-42665]
Immunoprecipitation: Pygopus-1 Antibody [NBP1-42665] - Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Affinity purified rabbit anti-Pygo1 antibody used for IP at 3 mcg/mg lysate. Pygo1 was also immunoprecipitated by rabbit anti-Pygo1 antibody NBP1-42666, which recognizes a downstream epitope.Applications for Pygopus-1 Antibody - BSA Free
Application
Recommended Usage
Immunohistochemistry
1:500 - 1:2000
Immunohistochemistry-Paraffin
1:500 - 1:2000
Immunoprecipitation
2-5 ug/mg lysate
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: Pygopus-1
Additional Pygopus-1 Products
Product Documents for Pygopus-1 Antibody - BSA Free
Product Specific Notices for Pygopus-1 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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