PTK9 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-90297
![Immunohistochemistry-Paraffin: PTK9 Antibody [NBP1-90297]](https://resources.bio-techne.com/images/products/PTK9-Antibody-Immunohistochemistry-Paraffin-NBP1-90297-img0014.jpg "Immunohistochemistry-Paraffin: PTK9 Antibody [NBP1-90297]")
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Simple Western
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: AEEELRQIKINEVQTDVGVDTKHQTLQGVAFPISREAFQALEKLNNRQLNYVQLEIDIKNEIIILANTTNTELKDLPKRIPKDSARYHFFLYKHSHEGDY
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
Novus Biologicals Rabbit PTK9 Antibody - BSA Free (NBP1-90297) is a polyclonal antibody validated for use in IHC, WB, ICC/IF and Simple Western. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for PTK9 Antibody - BSA Free
![Immunocytochemistry/ Immunofluorescence: PTK9 Antibody [NBP1-90297]](https://resources.bio-techne.com/images/products/PTK9-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-90297-img0012.jpg "Immunocytochemistry/ Immunofluorescence: PTK9 Antibody [NBP1-90297]")
Immunocytochemistry/ Immunofluorescence: PTK9 Antibody [NBP1-90297]
Immunocytochemistry/Immunofluorescence: PTK9 Antibody [NBP1-90297] - Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.![Immunohistochemistry-Paraffin: PTK9 Antibody [NBP1-90297]](https://resources.bio-techne.com/images/products/PTK9-Antibody-Immunohistochemistry-Paraffin-NBP1-90297-img0016.jpg "Immunohistochemistry-Paraffin: PTK9 Antibody [NBP1-90297]")
Immunohistochemistry-Paraffin: PTK9 Antibody [NBP1-90297]
Immunohistochemistry-Paraffin: PTK9 Antibody [NBP1-90297] - Staining of human urinary bladder shows moderate cytoplasmic positivity in urothelial cells.![Immunohistochemistry-Paraffin: PTK9 Antibody [NBP1-90297]](https://resources.bio-techne.com/images/products/PTK9-Antibody-Immunohistochemistry-Paraffin-NBP1-90297-img0013.jpg "Immunohistochemistry-Paraffin: PTK9 Antibody [NBP1-90297]")
Immunohistochemistry-Paraffin: PTK9 Antibody [NBP1-90297]
Immunohistochemistry-Paraffin: PTK9 Antibody [NBP1-90297] - Staining of human colon shows moderate cytoplasmic positivity in glandular cells.![Immunohistochemistry-Paraffin: PTK9 Antibody [NBP1-90297]](https://resources.bio-techne.com/images/products/PTK9-Antibody-Immunohistochemistry-Paraffin-NBP1-90297-img0015.jpg "Immunohistochemistry-Paraffin: PTK9 Antibody [NBP1-90297]")
![Simple Western: PTK9 Antibody [NBP1-90297]](https://resources.bio-techne.com/images/products/PTK9-Antibody-Simple-Western-NBP1-90297-img0008.jpg "Simple Western: PTK9 Antibody [NBP1-90297]")
![PTK9 Antibody - BSA Free Western Blot: PTK9 Antibody - BSA Free [NBP1-90297]](https://resources.bio-techne.com/images/products/nbp1-90297_rabbit-polyclonal-ptk9-antibody-29520251610104.jpg "Western Blot: PTK9 Antibody - BSA Free [NBP1-90297]")
![PTK9 Antibody - BSA Free Western Blot: PTK9 Antibody - BSA Free [NBP1-90297]](https://resources.bio-techne.com/images/products/nbp1-90297_rabbit-polyclonal-ptk9-antibody-30520257234220.jpg "Western Blot: PTK9 Antibody - BSA Free [NBP1-90297]")
Applications for PTK9 Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:200 - 1:500
Immunohistochemistry-Paraffin
1:200 - 1:500
Simple Western
1:40
Western Blot
0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF,Fixation Permeabilization: Use PFA/Triton X-100.
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
See Simple Western Antibody Database for Simple Western validation: Tested in RT-4 and U-251MG, separated by Size, antibody dilution of 1:40, apparent MW was 45 kDa. Separated by Size-Wes, Sally Sue/Peggy Sue.
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
See Simple Western Antibody Database for Simple Western validation: Tested in RT-4 and U-251MG, separated by Size, antibody dilution of 1:40, apparent MW was 45 kDa. Separated by Size-Wes, Sally Sue/Peggy Sue.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: PTK9
Alternate Names
A6, A6 protein tyrosine kinase, Protein A6, Protein tyrosine kinase 9MGC23788, PTK9 protein tyrosine kinase 9, PTK9MGC41876, twinfilin, actin-binding protein, homolog 1 (Drosophila), twinfilin-1
Gene Symbol
TWF1
Additional PTK9 Products
Product Documents for PTK9 Antibody - BSA Free
Product Specific Notices for PTK9 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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