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PSMD11 Antibody (2C7) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-59484

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 2C7

Format

BSA Free

Concentration

1 mg/ml

Product Specifications

Immunogen

Recombinant human PSMD11 (1-422aa) purified from E. coli

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Scientific Data Images for PSMD11 Antibody (2C7) - BSA Free

Western Blot: PSMD11 Antibody (2C7)BSA Free [NBP2-59484]

Western Blot: PSMD11 Antibody (2C7)BSA Free [NBP2-59484]

Western Blot: PSMD11 Antibody (2C7) [NBP2-59484] - Lane 1: HeLa cell lysates, Lane 2: 293T cell lysates, Lane 3: U87-MG cell lysates, Lane 4: NIH-3T3 cell lysates, Lane 5: PC3 cell lysates, Lane 6: TF1 cell lysates, Lane 7: MCF7 cell lysates
Immunocytochemistry/ Immunofluorescence: PSMD11 Antibody (2C7) - BSA Free [NBP2-59484]

Immunocytochemistry/ Immunofluorescence: PSMD11 Antibody (2C7) - BSA Free [NBP2-59484]

Immunocytochemistry/Immunofluorescence: PSMD11 Antibody (2C7) [NBP2-59484] - Analysis of PSMD11 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human PSMD11 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).
PSMD11 Antibody (2C7) - BSA Free

Western Blot: PSMD11 Antibody (2C7) - BSA Free [NBP2-59484] -

Simulated microgravity (sug) enhances PSMD11 expression in hPSCs.A Representative immunofluorescent micrographs of hPSCs cultured in sμg and 1g conditions and stained for PSMD11 and SOX2. DAPI was used to stain the nuclei of all cells. Scale bars, 200 um. Graphs showing the mean fluorescence intensity analysis from micrographs. B Representative Immunoblots and densitometry analysis of PSMD11 from protein lysates of cells cultured at sμg and 1g conditions. GAPDH was used as a loading control. Graphs on the right show the densitometry analysis of the immunoblots. C RT-qPCR analysis indicating the relative mRNA levels of PSMD11 present in cells cultured at sμg compared to 1g condition. *p < 0.05 (n = 3; unpaired t test). Error bars in graphs represent the SEM of the group. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35787634), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for PSMD11 Antibody (2C7) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4), 10% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PSMD11

PSMD11 (Proteasome 26S non-ATPase subunit 11), also known as S9, Rpn6 or p44.5, has multi-subunit protease complexes consisting of 20S subunits composed of four seven-numbered rings with two outer rings containing alpha subunits and two central rings composed of beta subunits, and 19S caps of 6 ATPase and 11 non-ATPase subunits. PSMD11 is the main proteolytic enzyme that functions in ATP-dependent degradation of ubiquitinated proteins.

Alternate Names

MGC3844,26S proteasome regulatory subunit RPN6, p44.5,26S proteasome regulatory subunit 9, proteasome (prosome, macropain) 26S subunit, non-ATPase, 11,26S proteasome regulatory subunit p44.5, Rpn6,26S proteasome regulatory subunit S9, S9,26S proteasome non-ATPase regulatory subunit 11

Gene Symbol

PSMD11

Additional PSMD11 Products

Product Documents for PSMD11 Antibody (2C7) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for PSMD11 Antibody (2C7) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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