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Protocadherin-15 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-94575

Novus Biologicals, part of Bio-Techne
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NBP2-94575-0.1ml
NBP2-94575-0.02ml

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 160-400 of human PCDH15 (NP_001136235.1). TVNELTPVGTTIFTGFSGDNGATDIDDGPNGQIEYVIQYNPDDPTSNDTFEIPLMLTGNIVLRKRLNYEDKTRYFVIIQANDRAQNLNERRTTTTTLTVDVLDGDDLGPMFLPCVLVPNTRDCRPLTYQAAIPELRTPEELNPIIVTPPIQAIDQDRNIQPPSDRPGILYSILVGTPEDYPRFFHMHPRTAELSLLEPVNRDFHQKFDLVIKAEQDNGHPLPAFAGLHIEILDENNQSPYF

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

216 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Rabbit Protocadherin-15 Antibody - BSA Free (NBP2-94575) is a polyclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for Protocadherin-15 Antibody - BSA Free

Western Blot: Protocadherin-15 AntibodyBSA Free [NBP2-94575]

Western Blot: Protocadherin-15 AntibodyBSA Free [NBP2-94575]

Western Blot: Protocadherin-15 Antibody [NBP2-94575] - Analysis of extracts of various cell lines, using Protocadherin-15 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 5s.

Applications for Protocadherin-15 Antibody - BSA Free

Application
Recommended Usage

ELISA

Recommended starting concentration is 1 μg/mL.

Immunohistochemistry

1:100-1:200

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Protocadherin-15

The PCDH15 gene is a member of the cadherin superfamily. Family members encode integral membrane proteins that mediatecalcium-dependent cell-cell adhesion. It plays an essential role in maintenance of normal retinal and cochlearfunction. Mutations in this gene

Alternate Names

CDHR15, DFNB23, PCDH15, Protocadherin15, USH1F

Gene Symbol

PCDH15

Additional Protocadherin-15 Products

Product Documents for Protocadherin-15 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Protocadherin-15 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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