Proprotein Convertase 9/PCSK9 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB300-959

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NB300-959
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Peptide ELISA, Immunocytochemistry/ Immunofluorescence, Chromatin Immunoprecipitation (ChIP)

Cited:

Western Blot, Immunocytochemistry/ Immunofluorescence, Chemotaxis

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all Proprotein Convertase 9/PCSK9 Antibodies »

Product Specifications

Immunogen

Peptide with sequence CRSRHLAQASQELQ corresponding to C-Terminus according to NP_777596.2.

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 29593095).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Proprotein Convertase 9/PCSK9 Antibody

Western Blot: Proprotein Convertase 9/PCSK9 Antibody [NB300-959]

Western Blot: Proprotein Convertase 9/PCSK9 Antibody [NB300-959]

Western Blot: Proprotein Convertase 9/PCSK9 Antibody [NB300-959] - (0.3ug/ml) staining of Human Adipose lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: Proprotein Convertase 9/PCSK9 Antibody [NB300-959]

Immunohistochemistry-Paraffin: Proprotein Convertase 9/PCSK9 Antibody [NB300-959]

Immunohistochemistry-Paraffin: Proprotein Convertase 9/PCSK9 Antibody [NB300-959] - Negative Control showing staining of paraffin embedded Human Liver, with no primary antibody.
Immunohistochemistry-Paraffin: Proprotein Convertase 9/PCSK9 Antibody [NB300-959]

Immunohistochemistry-Paraffin: Proprotein Convertase 9/PCSK9 Antibody [NB300-959]

Immunohistochemistry-Paraffin: Proprotein Convertase 9/PCSK9 Antibody [NB300-959] - (5ug/ml) staining of paraffin embedded Human Liver. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.

Applications for Proprotein Convertase 9/PCSK9 Antibody

Application
Recommended Usage

Chromatin Immunoprecipitation (ChIP)

1:10-1:500

Immunohistochemistry-Paraffin

5-6 ug/ml

Peptide ELISA

detection limit 1:16000

Western Blot

0.3-1 ug/ml
Application Notes
ICC/IF reactivity reported in (PMID: 30210081), Chip reactivity reported in (PMID: 20427574).

Reviewed Applications

Read 1 review rated 4 using NB300-959 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Proprotein Convertase 9/PCSK9

This gene encodes a proprotein convertase belonging to the proteinase K subfamily of the secretory subtilase family. The encoded protein is synthesized as a soluble zymogen that undergoes autocatalytic intramolecular processing in the endoplasmic reticulum. The protein may function as a proprotein convertase. This protein plays a role in cholesterol homeostasis and may have a role in the differentiation of cortical neurons. Mutations in this gene have been associated with a third form of autosomal dominant familial hypercholesterolemia (HCHOLA3).

Alternate Names

FH3, FHCL3, HCHOLA3, LDLCQ1, NARC-1, NARC1, PC9, PCSK9

Entrez Gene IDs

255738 (Human)

Gene Symbol

PCSK9

UniProt

Q8NBP7

Additional Proprotein Convertase 9/PCSK9 Products

Product Documents for Proprotein Convertase 9/PCSK9 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Proprotein Convertase 9/PCSK9 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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