Prohibitin Antibody (0V2R10)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15756
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 0V2R10 expressed in HEK293
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 100-200 of human Prohibitin (P35232). ASQLPRIFTSIGEDYDERVLPSITTEILKSVVARFDAGELITQRELVSRQVSDDLTERAATFGLILDDVSLTHLTFGKEFTEAVEAKQVAQQEAERARFVV
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
30 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Novus Biologicals Rabbit Prohibitin Antibody (0V2R10) (NBP3-15756) is a recombinant monoclonal antibody validated for use in IHC, WB and ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for Prohibitin Antibody (0V2R10)
![Western Blot: Prohibitin Antibody (0V2R10) [NBP3-15756]](https://resources.bio-techne.com/images/products/Prohibitin-Antibody-0V2R10-Western-Blot-NBP3-15756-img0004.jpg "Western Blot: Prohibitin Antibody (0V2R10) [NBP3-15756]")
Western Blot: Prohibitin Antibody (0V2R10) [NBP3-15756]
Western Blot: Prohibitin Antibody (0V2R10) [NBP3-15756] - Western blot analysis of extracts of various cell lines, using Prohibitin antibody (NBP3-15756) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.
Immunohistochemistry: Prohibitin Antibody (0V2R10) [NBP3-15756] -
Immunohistochemistry: Prohibitin Antibody (0V2R10) [NBP3-15756] - Immunohistochemistry analysis of Prohibitin in paraffin-embedded mouse kidney tissue using Prohibitin Rabbit mAb at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry: Prohibitin Antibody (0V2R10) [NBP3-15756] -
Immunohistochemistry: Prohibitin Antibody (0V2R10) [NBP3-15756] - Immunohistochemistry analysis of Prohibitin in paraffin-embedded human colon carcinoma tissue using Prohibitin Rabbit mAb at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Applications for Prohibitin Antibody (0V2R10)
Application
Recommended Usage
ELISA
Recommended starting concentration is 1 μg/mL
Immunohistochemistry
1:50 - 1:200
Immunohistochemistry-Paraffin
1:50 - 1:200
Western Blot
1:1000 - 1:4000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol, 0.05% BSA
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Prohibitin
Alternate Names
PHB
Gene Symbol
PHB1
Additional Prohibitin Products
Product Documents for Prohibitin Antibody (0V2R10)
Product Specific Notices for Prohibitin Antibody (0V2R10)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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