PPAR gamma/NR1C3 Antibody - BSA Free

Western Blot: PPAR gamma/NR1C3 Antibody [NB120-19481]

PPAR-gamma-NR1C3-Antibody-Western-Blot-NB120-19481-img0001.jpg

Western Blot: PPAR gamma/NR1C3 Antibody [NB120-19481] -

Western Blot: PPAR gamma/NR1C3 Antibody [NB120-19481] - Dnmt3a1 decreases lipid droplet accumulation in the early stage of adipocyte differentiation. (A) Representative images of oil red O staining (red) & DAPI staining (blue) after transfection with pSDS-Dnmt3a1 & pSDS-NC for 48 h are showed; scale bar: 25 μm. (B) Lipid droplet content by oil red O staining & extraction method of cells transfected with pSDS-Dnmt3a1 & pSDS-NC for 48 h. Values represent mean ± SEM from three independent experiments. ∗∗P < 0.01. (C) Representative images of oil red O staining (red) & DAPI staining (blue) after transfection with si-Dnmt3a1 & si-NC for 48 h are showed; scale bar: 25 μm. (D) Lipid droplet content by oil red O staining & the extraction method of cells transfected with si-Dnmt3a1 & si-NC for 48 h. Values represent mean ± SEM from three independent experiments. ∗P < 0.05. (E) Relative mRNA level of some general genes implicated in adipogenesis or energy homeostasis induced by pSDS-Dnmt3a1 & pSDS-NC into cells. Values represent mean ± SEM from four separate experiments. ∗P < 0.05, ∗∗P < 0.01, & ∗∗∗P < 0.001. (F) Protein expression of some general adipose genes induced by pSDS-Dnmt3a1 & pSDS-NC into cells. (G) Relative mRNA level of some general genes involved in adipogenesis or energy homeostasis after transfection with si-Dnmt3a1 & si-NC into cells for 48 h. Values represent mean ± SEM from four separate experiments. ∗P < 0.05, ∗∗P < 0.01, & ∗∗∗P < 0.001. (H) Protein expression of some general genes involved in adipogenesis at 48 h after transfection by si-Dnmt3a1 & si-NC into cells. The band intensity of Western blotting was obtained by averaging the data from three independent experiments. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30333755), licensed under a CC-BY license. Not internally tested by Novus Biologicals.