PP4/PPP4C Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-13802
![Immunohistochemistry-Paraffin: PP4/PPP4C Antibody [NBP2-13802]](https://resources.bio-techne.com/images/products/PPP4C-Antibody-Immunohistochemistry-Paraffin-NBP2-13802-img0007.jpg "Immunohistochemistry-Paraffin: PP4/PPP4C Antibody [NBP2-13802]")
![Immunohistochemistry-Paraffin: PP4/PPP4C Antibody [NBP2-13802]](https://resources.bio-techne.com/images/products/PPP4C-Antibody-Immunohistochemistry-Paraffin-NBP2-13802-img0006.jpg "Immunohistochemistry-Paraffin: PP4/PPP4C Antibody [NBP2-13802]")
![Immunohistochemistry-Paraffin: PP4/PPP4C Antibody [NBP2-13802]](https://resources.bio-techne.com/images/products/PPP4C-Antibody-Immunohistochemistry-Paraffin-NBP2-13802-img0008.jpg "Immunohistochemistry-Paraffin: PP4/PPP4C Antibody [NBP2-13802]")
Key Product Details
Validated by
Orthogonal Validation
Species Reactivity
Validated:
Human
Predicted:
Mouse (100%), Rat (100%). Backed by our 100% Guarantee.
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against a recombinant protein corresponding to the amino acids: MAEISDLDRQIEQLRRCELIKESEVKALCAKAREILVEESNVQRVDSPVTVCG
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
Novus Biologicals Rabbit PP4/PPP4C Antibody - BSA Free (NBP2-13802) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for PP4/PPP4C Antibody - BSA Free
![Western Blot: PP4/PPP4C Antibody [NBP2-13802]](https://resources.bio-techne.com/images/products/PPP4C-Antibody-Western-Blot-NBP2-13802-img0004.jpg "Western Blot: PP4/PPP4C Antibody [NBP2-13802]")
Western Blot: PP4/PPP4C Antibody [NBP2-13802]
Western Blot: PP4/PPP4C Antibody [NBP2-13802] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4![Immunocytochemistry/ Immunofluorescence: PP4/PPP4C Antibody [NBP2-13802]](https://resources.bio-techne.com/images/products/PPP4C-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-13802-img0005.jpg "Immunocytochemistry/ Immunofluorescence: PP4/PPP4C Antibody [NBP2-13802]")
Immunocytochemistry/ Immunofluorescence: PP4/PPP4C Antibody [NBP2-13802]
Immunocytochemistry/Immunofluorescence: PP4/PPP4C Antibody [NBP2-13802] - Staining of human cell line U-2 OS shows localization to nucleus, plasma membrane & cytosol. Antibody staining is shown in green.Applications for PP4/PPP4C Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:2500 - 1:5000
Immunohistochemistry-Paraffin
1:2500 - 1:5000
Western Blot
0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. For ICC/IF Fixation/Permeabilization: PFA/Triton X-100.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: PP4
PP1 (Protein Phosphatase 1), also known as PPP1, is an enzyme that removes phosphate groups from serine or threonine residues. The holoenzyme is composed of two subunits. The catalytic subunit is highly conserved throughout evolution, and interacts with a wide range of proteins acting as regulatory subunits that target the enzyme to specific subcellular compartments and substrates. The catalytic subunit is ubiquitously expressed in tissues and cell lines.
Long Name
Protein Phosphatase 4
Alternate Names
PP4C, PPH3, PPP4C, PPX
Gene Symbol
PPP4C
Additional PP4 Products
Product Documents for PP4/PPP4C Antibody - BSA Free
Product Specific Notices for PP4/PPP4C Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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